Construct: ORF TRCN0000467230
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008475.1_s317c1
- Derived from:
- ccsbBroadEn_12162
- DNA Barcode:
- TTGATTCCCGCAGGTTAACACAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF38B (55119)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467230
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55119 | PRPF38B | pre-mRNA processing factor 38B | XM_011541687.3 | 92.9% | 91.6% | (many diffs) |
| 2 | human | 55119 | PRPF38B | pre-mRNA processing factor 38B | NM_018061.4 | 34.1% | 34% | 559_560delGAinsTTCTTCAC;564_1638delinsC |
| 3 | human | 55119 | PRPF38B | pre-mRNA processing factor 38B | NM_001349757.2 | 13.8% | 13.7% | 0_1ins333;226_227delGAinsTTCTTCAC;231_1305delinsC |
| 4 | human | 55119 | PRPF38B | pre-mRNA processing factor 38B | XR_001737264.2 | 12.7% | (many diffs) | |
| 5 | human | 55119 | PRPF38B | pre-mRNA processing factor 38B | XR_001737263.2 | 12.5% | (many diffs) | |
| 6 | mouse | 66921 | Prpf38b | PRP38 pre-mRNA processing f... | NM_025845.2 | 32% | 33.9% | (many diffs) |
| 7 | mouse | 66921 | Prpf38b | PRP38 pre-mRNA processing f... | XR_001783735.1 | 16.7% | (many diffs) | |
| 8 | mouse | 66921 | Prpf38b | PRP38 pre-mRNA processing f... | XM_006501914.1 | 13.3% | 13.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 636
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc taacaacagc cccgcgctga caggcaactc gcagccgcag caccaggcgg 121 ctgcagctgc ggctcagcaa cagcagcagt gcggcggcgg cggcgctacc aagccggcgg 181 tctccggcaa gcagggcaat gtgctcccgc tctggggcaa cgagaagacc atgaacctca 241 accccatgat cctgaccaac atcctgtcgt cgccttactt caaagtacag ctctacgagc 301 tcaagaccTA CCACGAGGTG GTGGACGAGA TCTACTTTAA GGTCACGCAC GTTGAACCAT 361 GGGAGAAAGG AAGCAGGAAA ACAGCGGGCC AGACAGGGAT GTGCGGAGGG GTTCGAGGTG 421 TTGGAACAGG AGGAATTGTT TCTACAGCAT TTTGCCTGTT ATACAAATTA TTTACCCTGA 481 AGTTAACTCG AAAGCAAGTG ATGGGTCTTA TAACACACAC AGACTCTCCA TATATTAGAG 541 CGCTTGGATT TATGTATATA AGATATACAC AGCCCCCTAC AGATCTGTGG GACTGGTTTG 601 AATCCTTCCT TGATGATGAA GAGTTCTTCA CCCTCTACCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 ATTGATTCCC GCAGGTTAAC ACAGAACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt