Construct: ORF TRCN0000467248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002526.2_s317c1
- Derived from:
- ccsbBroadEn_09137
- DNA Barcode:
- TACCCGACAGTCGCGCTCAACCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSSK1B (83942)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467248
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83942 | TSSK1B | testis specific serine kina... | NM_032028.4 | 99.9% | 100% | 1098C>A |
| 2 | mouse | 22114 | Tssk1 | testis-specific serine kina... | NM_009435.2 | 86% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1167
- ORF length:
- 1101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgacgctgct gtcctcaagc gacgaggcta cctcctgggg ataaatttag 121 gagagggctc ctatgcaaaa gtaaaatctg cttactctga gcgcctgaag ttcaatgtgg 181 cgatcaagat catcgaccgc aagaaggccc ccgcagactt cttggagaaa ttccttcccc 241 gggaaattga gattctggcc atgttaaacc actgctccat cattaagacc tacgagatct 301 ttgagacatc acatggcaag gtctacatcg tcatggagct cgcggtccag ggcgacctcc 361 tcgagttaat caaaacccgg ggagccctgc atgaggacga agctcgcaag aagttccacc 421 agctttcctt ggccatcaag tactgccacg acctggacgt cgtccaccgg gacctcaagt 481 gtgacaacct tctccttgac aaggacttca acatcaagct gtccgacttc agcttctcca 541 agcgctgcct gcgggatgac agtggtcgaa tggcattaag caagaccttc tgtgggtcac 601 cagcgtatgc ggccccagag gtgctgcagg gcattcccta ccagcccaag gtgtacgaca 661 tctggagcct aggcgtgatc ctctacatca tggtctgcgg ctccatgccc TACGACGACT 721 CCAACATCAA GAAGATGCTG CGTATCCAGA AGGAGCACCG CGTCAACTTC CCACGCTCCA 781 AGCACCTGAC AGGCGAGTGC AAGGACCTCA TCTACCACAT GCTGCAGCCC GACGTCAACC 841 GGCGGCTCCA CATCGACGAG ATCCTCAGCC ACTGCTGGAT GCAGCCCAAG GCACGGGGAT 901 CTCCCTCTGT GGCCATCAAC AAGGAGGGGG AGAGTTCCCG GGGAACTGAA CCCTTGTGGA 961 CCCCCGAACC TGGCTCTGAC AAGAAGTCTG CCACCAAGCT GGAGCCTGAG GGAGAGGCAC 1021 AGCCCCAGGC ACAGCCTGAG ACAAAACCCG AGGGGACAGC AATGCAAATG TCCAGGCAGT 1081 CGGAGATCCT GGGTTTCCCC AGCAAGCCGT CGACTATGGA GACAGAGGAA GGGCCCCCCC 1141 AACAGCCTCC AGAGACGCGG GCACAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATACCCGAC 1321 AGTCGCGCTC AACCGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1381 aagatt