Construct: ORF TRCN0000467259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003753.1_s317c1
- Derived from:
- BRDN0000395072
- DNA Barcode:
- GCCAGATTTTCCCCACATAAGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CARD11 (84433)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467259
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84433 | CARD11 | caspase recruitment domain ... | NM_001324281.2 | 99.1% | 99% | (many diffs) |
| 2 | human | 84433 | CARD11 | caspase recruitment domain ... | NM_032415.6 | 99.1% | 99% | (many diffs) |
| 3 | mouse | 108723 | Card11 | caspase recruitment domain ... | NM_175362.2 | 86.2% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3510
- ORF length:
- 3441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgactgc atggagacgc tggtgcatga agaggacgcc ttgtgggaga 121 atgtggagtg taaccggcac atgctcagcc gctatatcaa ccctgccaag ctcacgccct 181 acctgcgtca gtgtaaggtc attgatgagc aggatgaaga tgaagtgctt aatgccccta 241 tgctgccatc caagatcaac cgagcaggcc ggctgttgga cattctacat accaaggggc 301 aaaggggcta tgtggtcttc ttggagagcc tagaatttta ttacccagaa ctgtacaaac 361 tggtgactgg gaaagagccc actcggagat tctccaccat tgtggtggag gaaggccacg 421 agggcctcac gcacttcctg atgaacgagg tcatcaagct gcagcagcag atgaaggcca 481 aggacctgca acgctgcgag ctgctggcca ggttgcggca gctggaggat gagaagaagc 541 agatgacgct gacgcgcgtg gagctgctaa ccttccagga gcggtactac aagatgaagg 601 aagagcggga cagctacaat gacgagctgg tcaaggtgaa ggacgacaac tacaacttag 661 ccatgcgcta cgcacagctc agtgaggaga agaacatggc ggtcatgagg agccgagacc 721 tccaactcga gatcgatcag ctaaagcacc ggttgaataa gatggaggag gaatgtaagc 781 tggagagaaa tcagtctcta aaactgaaga atgacattga aaatcggccc aagaaggagc 841 aggttctgga actggagcgg gagaatgaaa tgctgaagac caaaaaccag gagctgcagt 901 ccatcatcca ggccgggaag cgcagcctgc cagactcaga caaggccatc ctggacatct 961 tggaacacga ccgcaaggag gccctggagg acaggcagga gctggtcaac aggatctaca 1021 acctgcagga ggaggcccgc caggcagagg agctgcgaga caagtacctg gaggagaagg 1081 aggacctgga gctcaagtgc tcgaccctgg gaaaggactg tgaaatgtac aagcaccgca 1141 tgaacacggt catgctgcag ctggaggagg tggagcggga gcgggaccag gccttccact 1201 cccgagatga agctcagaca cagtactcgc agtgcttaat cgaaaaggac aagtacagga 1261 agcagatccg cgagctggag gagaagaacg atgagatgag gatcgagatg gtgcggcggg 1321 aggcctgcat cgtcaacctg gagagcaagc tgcggcgcct ctccaaggac agcaacaacc 1381 tggaccagag tctgcccagg aacctgccag taaccatcat ctctcaggac tttggggatg 1441 ccagccccag gaccaatggt caagaagctg acgattcttc cacctcggag gagtcacctg 1501 aagacagcaa gtacttcctg ccctaccatc cgccccagcg caggatgaac ctgaagggca 1561 tccagctgca gagagccaaa tcccccatca gcctgaagcg aacatcagat tttcaagcca 1621 aggggcacga ggaagaaggc acggatgcca gccctagctc ctgcggatct ctgcccatca 1681 ccaactcctt caccaagatg cagccccccc ggagccgcag cagcatcatg tcaatcaccg 1741 ccgagccccc gggaaacgac tccatcgtca gacgctacaa ggaggacgcg ccccatcgca 1801 gcacagtcga agaagacaat gacagcggcg ggtttgacgc cttagatctg gatgatgaca 1861 gtcacgaacg ctactccttc ggaccctcct ccatccactc ctcctcctcc tcccaccaat 1921 ccgagggcct ggatgcctac gacctggagc aggtcaacct catgttcagg aagttctctc 1981 tggaaagacc cttccggcct tcggtcacct ctgtggggca cgtgcggggc ccagggccct 2041 cggtgcagca cacgacgctg aatggcgaca gcctcacctc ccagctcacc ctgctggggg 2101 gcaacgcgcg agggagcttc gtgcactcgg tcaagcctgg ctctctggcc gagaaagccg 2161 gcctccgtga gggccaccag ctgctgctgc tagaaggctg catccgaggc gagaggcaga 2221 gtgtcccgtt ggacacatgc accaaagagg aagcccactg gaccatccag aggtgcagcg 2281 gccccgtcac gctgcactac aaggtcaacc acgaagggta ccggaagctg gtgaaggaca 2341 tggaggacgg cctgatcaca tcgggggact cgttctacat ccggctgaac ctgaacatct 2401 ccagccagct ggacgcctgc accatgtccc tgaagtgtga cgatgttgtg cacgtccgtg 2461 acaccatgta ccaggacagg cacgagtggc tgtgcgcgcg ggtcgaccct ttcacagacc 2521 atgacctgga tatgggcacc atacccagct acagccgagc ccagcagctc ctcctggtga 2581 aactgcagcg cctgatgcac cgaggcagcc gggaggaggt agacggcacc caccacaccc 2641 tgcgggcact ccggaacacc ctgcagccag aagaagcgct ttcaacaagc gacccccggg 2701 tcagcccccg tctctcgcga gcaagcttcc tttttggcca gctccttcag ttcgtcagca 2761 ggtccgagaa caagtataag cggatgaaca gcaacgagcg ggtccgcatc atctcgggga 2821 gtccgctagg gagcctggcc cggtcctcgc tggacgccac caagctcttg actgagaagc 2881 aggaagagct ggaccctgag agcgagctgg gcaagaacct cagcctcatc ccctacagcc 2941 tggtacgcgc cttctactgc gagcgccgcc ggcccgtgct cttcacaccc accgtgctgg 3001 cggagacgct ggtgcagagg ctgctcaact cgggaggtgc catggagttc accatctgca 3061 agtcagatat cgtcacaaga gatgagttcc TCAGAAGGCA GAAGACGGAG ACCATCATCT 3121 ACTCCCGAGA GAAGAACCCC AACGCGTTCG AATGCATCGC CCCTGCCAAC ATTGAAGCTG 3181 TGGCCGCCAA GAACAAGCAC TGCCTGCTGG AGGCTGGGAT CGGCTGCACA AGAGACTTGA 3241 TCAAGTCCAA CATCTACCCC ATCGTGCTCT TCATCCGGGT GTGTGAGAAG AACATCAAGA 3301 GGTTCAGAAA GCTGCTGCCC CGACCTGAGA CGGAGGAGGA GTTCCTGCGC GTGTGCCGGC 3361 TGAAGGAGAA GGAGCTGGAG GCCCTGCCGT GCCTGTACGC CACGGTGGAA CCTGACATGT 3421 GGGGCAGCGT AGAGGAGCTG CTCCGCGTTG TCAAGGACAA GATCGGCGAG GAGCAGCGCA 3481 AGACCATCTG GGTGGACGAG GACCAGCTGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 3541 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3601 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCCAG 3661 ATTTTCCCCA CATAAGCACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3721 tgaaagatt