Construct: ORF TRCN0000467263
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008277.1_s317c1
- Derived from:
- ccsbBroadEn_10568
- DNA Barcode:
- GAAGACACTCTCCAACTGCCGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN5P1 (155400)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467263
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168348.2 | 39.3% | 38.1% | (many diffs) |
| 2 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_018044.4 | 35.8% | 34.7% | (many diffs) |
| 3 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_148956.4 | 32.9% | 31.9% | (many diffs) |
| 4 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168347.3 | 32.6% | 31.7% | (many diffs) |
| 5 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XR_242255.5 | 28.3% | (many diffs) | |
| 6 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_033322.3 | 27.2% | 1_973del;1109_1116delGGTGAGAT;1469_1786delinsCG | |
| 7 | human | 260294 | NSUN5P2 | NSUN5 pseudogene 2 | NR_033323.3 | 27.1% | (many diffs) | |
| 8 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_104013.1 | 25.8% | (many diffs) | |
| 9 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XM_005277604.5 | 25.7% | 10.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 558
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccacgctg ctggcctggg ttggcgtctc ctgctgtgag ctggctgagg 121 aggacttcct ggcggtctcc cccttagatc cgcgctatcg tgaggTCCAC TATGTCCTGC 181 TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAT CCCGGGGCAG 241 GGACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA GCCCTGTGCC 301 ACGCGCTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CATGTGCTCC CTCTGCCAGG 361 AGGAGAATGA AGACATGGTA CCAGATGCGC TGCAGCAGAA CCCGGGCGCC TTCAGGCTAG 421 CTCCCGCCCT GCCTGCCCGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT GCCGAGCACT 481 GCCTCCGGGC TTCCCCCAAG ACCACGCTTA GCGGTGGCTT CTTCGTTGCT GTAATTGAAC 541 GGGTCGAGAT GCCGACGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAAGACA CTCTCCAACT 721 GCCGTTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt