Construct: ORF TRCN0000467265
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012844.1_s317c1
- Derived from:
- ccsbBroadEn_01159
- DNA Barcode:
- AACTCATGCTGGTCAACCACTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE6G (5148)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467265
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5148 | PDE6G | phosphodiesterase 6G | NM_001365724.1 | 100% | 100% | |
| 2 | human | 5148 | PDE6G | phosphodiesterase 6G | NM_001365725.1 | 100% | 100% | |
| 3 | human | 5148 | PDE6G | phosphodiesterase 6G | NM_002602.4 | 100% | 100% | |
| 4 | human | 5148 | PDE6G | phosphodiesterase 6G | XM_017024736.1 | 100% | 100% | |
| 5 | human | 5148 | PDE6G | phosphodiesterase 6G | XM_006721942.3 | 63.5% | 63.5% | 1_150del |
| 6 | human | 5148 | PDE6G | phosphodiesterase 6G | XM_017024734.1 | 55.4% | 55.4% | 1_210del |
| 7 | human | 5148 | PDE6G | phosphodiesterase 6G | NR_158591.1 | 18.4% | (many diffs) | |
| 8 | human | 5148 | PDE6G | phosphodiesterase 6G | NR_026872.2 | 17.1% | (many diffs) | |
| 9 | mouse | 18588 | Pde6g | phosphodiesterase 6G, cGMP-... | NM_012065.3 | 89.6% | 96.5% | (many diffs) |
| 10 | mouse | 18588 | Pde6g | phosphodiesterase 6G, cGMP-... | XM_006532455.3 | 89.6% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 354
- ORF length:
- 261
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAACCT GGAACCGCCC AAGGCTGAGT 121 TCCGGTCAGC CACCAGGGTG GCCGGGGGAC CTGTCACCCC CAGGAAAGGG CCCCCTAAAT 181 TTAAGCAGCG ACAGACCAGG CAGTTCAAGA GCAAGCCCCC AAAGAAAGGC GTTCAAGGGT 241 TTGGGGACGA CATCCCTGGA ATGGAAGGCC TGGGAACAGA CATCACAGTC ATCTGCCCTT 301 GGGAGGCCTT CAACCACCTG GAGCTGCACG AGCTGGCCCA ATATGGCATC ATCTTGCCAA 361 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 421 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 481 TATCTTGTGG AAAGGACGAA ACTCATGCTG GTCAACCACT TATACGCGTT AAGTCgacaa 541 tcaacctctg gattacaaaa tttgtgaaag att