Construct: ORF TRCN0000467286
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012114.1_s317c1
- Derived from:
- ccsbBroadEn_11400
- DNA Barcode:
- GAACAGGAATGCGAATTAAGGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDC3 (9672)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467286
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9672 | SDC3 | syndecan 3 | XM_011542466.1 | 88.1% | 82.9% | (many diffs) |
| 2 | human | 9672 | SDC3 | syndecan 3 | XM_011542464.2 | 84.5% | 77.3% | (many diffs) |
| 3 | human | 9672 | SDC3 | syndecan 3 | XM_011542463.1 | 83.9% | 77.1% | (many diffs) |
| 4 | human | 9672 | SDC3 | syndecan 3 | NM_014654.4 | 80.4% | 75.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cattgcttac ctgggatcct catgcccctc acaaccaccc agctccctag 121 ctctctccct ctcccccacc ccctcagact tcgagcagga gtcgggcatt gagacagcca 181 tgcgcttcag cccagatgta gccctggcgg tgtccaccac accatttgaa gagctcccct 241 ctgagcgccc caccctggag ccagccacca gccccctggt ggtgacagaa gtcccggaag 301 agcccagcca gagagccacc accgtctcca ctaccatggc taccactgct gccacaagca 361 caggggaccc gactgtggcc acagtgcctg ccacagtggc caccgccacc cccagcaccc 421 ctgcagcacc cccttttacg gccaccactg ctgttataag gaccactggc gtacggaggc 481 ttctgcctct cccactgacc acagtggcta cggcacgggc cactaccccc gaggcgccct 541 ccccgcccac cacggcggct gtcttggaca ccgaggcccc aacacccagg ctggtcagca 601 cagctacctc ccggccaaga gcccttccca ggccggccac cacccaggag cctgacatcc 661 ctgagaggag caccctgccc ctggggacca ctgcccctgg acccacagag gtggctcaga 721 ccccaactcc agagaccttc ctgaccacaa tccgggatga gccagaggtt ccggtgagtg 781 ggGGGCCCAG TGGAGACTTC GAGCTGCCAG AAGAAGAGAC CACACAACCA GACACAGCCA 841 ATGAGGTGGT AGCTGTGGGA GGGGCTGCGG CCAAGGCATC ATCTCCACCT GGGACACTGC 901 CCAAGGGTGC CCGCCCGGGC CCTGGCCTCC TGGACAATGC CATCGACTCG GGCAGCTCAG 961 CTGCTCAGCT GCCTCAGAAG AGTATCCTGG AGCGGAAGGA GGTGCTCGTA GCTGTGATTG 1021 TGGGCGGGGT GGTGGGCGCC CTCTTTGCTG CCTTCTTGGT CACACTGCTC ATCTATCGTA 1081 TGAAGAAAAA GGATGAGGGC AGCTACACGC TGGAGGAACC CAAGCAGGCG AGCGTCACAT 1141 ACCAGAAGCC TGACAAGCAG GAGGAGTTCT ATGCCTACCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 AGAACAGGAA TGCGAATTAA GGCAGACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt