Construct: ORF TRCN0000467290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003780.1_s317c1
- Derived from:
- ccsbBroadEn_15627
- DNA Barcode:
- TAGCGAGGCGCACCCTCTACTGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BSND (7809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7809 | BSND | barttin CLCNK type accessor... | NM_057176.3 | 53.3% | 53.4% | 513_960del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 579
- ORF length:
- 513
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgacgagaag accttccgga tcggcttcat tgtgctgggg cttttcctgc 121 tggccctcgg tacgttcctc atgagccatg atcggcccca ggtctacggc accttctatg 181 ccatgggcag cgtcatggtg atcgggggca tcatctggag catgtgccag tgctacccca 241 agatcacctt cgtccctgct gactctgact ttcaaggcat CCTCTCCCCA AAGGCCATGG 301 GCCTGCTGGA GAATGGGCTT GCTGCCGAGA TGAAGAGCCC CAGTCCCCAG CCGCCCTATG 361 TAAGGCTGTG GGAGGAAGCC GCCTATGACC AGAGCCTGCC TGACTTCAGC CACATCCAGA 421 TGAAAGTCAT GAGCTACAGT GAGGACCACC GCTCCTTGCT GGCCCCTGAG ATGGGGCAGC 481 CGAAGCTGGG AACCAGTGAT GGAGGAGAAG GTGGCCCTGG CGACGTTCAG GCCTGGATGG 541 AGGCTGCCGT GGTCATCCAC AAGGGCTCAG ACGAGAGTGC CTAACTTTCT TGTACAAAGT 601 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 661 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 721 CGATAGCGAG GCGCACCCTC TACTGATACG CGTTAAGTCg acaatcaacc tctggattac 781 aaaatttgtg aaagatt