Construct: ORF TRCN0000467295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014271.1_s317c1
- Derived from:
- ccsbBroadEn_03224
- DNA Barcode:
- ACCATCATGATTGCCCTGGGATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RLIM (51132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467295
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51132 | RLIM | ring finger protein, LIM do... | NM_016120.4 | 100% | 100% | |
| 2 | human | 51132 | RLIM | ring finger protein, LIM do... | NM_183353.3 | 100% | 100% | |
| 3 | mouse | 19820 | Rlim | ring finger protein, LIM do... | NM_011276.3 | 84% | 85.7% | (many diffs) |
| 4 | mouse | 19820 | Rlim | ring finger protein, LIM do... | XM_006527918.3 | 84% | 85.7% | (many diffs) |
| 5 | mouse | 19820 | Rlim | ring finger protein, LIM do... | XM_006527919.3 | 84% | 85.7% | (many diffs) |
| 6 | mouse | 19820 | Rlim | ring finger protein, LIM do... | XM_006527920.3 | 84% | 85.7% | (many diffs) |
| 7 | mouse | 19820 | Rlim | ring finger protein, LIM do... | XM_006527921.3 | 84% | 85.7% | (many diffs) |
| 8 | mouse | 19820 | Rlim | ring finger protein, LIM do... | XM_011247551.2 | 84% | 85.7% | (many diffs) |
| 9 | mouse | 19820 | Rlim | ring finger protein, LIM do... | XM_011247552.2 | 84% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1938
- ORF length:
- 1872
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaactcagat tccaatgaca aaggaagtgg tgatcagtct gcagcacagc 121 gcagaagtca gatggaccga ttggatcgag aagaagcttt ctatcaattt gtaaataacc 181 tgagtgaaga agattatagg cttatgagag ataacaattt gctaggcacc ccaggtgaaa 241 gtactgagga agagttgctg agacgactac agcaaattaa agaaggccca ccaccgcaaa 301 actcagatga aaatagagga ggagactctt cagatgatgt gtctaatggt gactctataa 361 tagactggct taactctgtc agacaaactg gaaatacaac aagaagtggg caaagaggaa 421 accaatcttg gagagcagtg agtcggacta atccaaacag tggtgatttc agattcagtt 481 tagagataaa tgttaaccgt aataatggga gccaaaattc agagaatgaa aatgagccat 541 ctgcaagacg ttctagtgga gaaaatgtgg aaaacaacag ccaaaggcaa gtggaaaacc 601 cacgatctga atcaacatct gcaaggccat ctagatcaga acgaaattca actgaagcgt 661 taacagaggt cccacctacc agaggtcaga ggagggcaag aagcaggagc ccagaccatc 721 ggagaaccag agcaagagct gaaagaagta ggtcacctct gcatccaatg agtgaaattc 781 cacgaagatc tcatcatagt atctcatctc agacttttga acatcctttg gtaaatgaga 841 cggagggaag ttctagaacc cggcaccatg tgacattgag gcagcaaata tctgggcctg 901 agttgctaag tagaggtctt tttgcagctt ctggaacaag aaatgcttct caaggagcag 961 gttcttcaga cacagctgcc agtggtgaat ctacaggatc aggacagaga cctccaacca 1021 tagtccttga tcttcaagta agaagagttc gtcctggaga atatcggcag agagatagca 1081 tagccagcag aactcggtct aggtctcaga caccaaacaa cactgtcacc tatgaaagtg 1141 aacgaggagg ttttaggcgt acattttcac gttctgagcg ggcaggtgtg agaacctatg 1201 tcagtaccat cagaattccc attcgtagaa tcttaaatac tggtttaagt gagactacat 1261 ctgttgcaat tcagaccatg ttaaggcaga taatgacagg ttttggtgag ttaagctatt 1321 ttatgtacag tgatagcgac tcagagccta ctggctcagt ctcaaatcga aatatggaaa 1381 gggcagagtc acggagtgga agaggaggtt ctggtggtgg tagtagttct ggttccagtt 1441 cgagttccag ttccagttcg agttccagtt ccagttcaag ttccagttcc agtcctagtt 1501 ccagttccgg tggtgaaagt tcagaaacta gctcagattt atttgaaggc agtaatgaag 1561 gaagctcatc atcaggctca tcaggtgcca ggcgagaggg tcgacatagg gccccagtca 1621 cattTGATGA AAGTGGCTCT TTGCCCTTCC TTAGCCTGGC TCAGTTTTTC CTCTTAAATG 1681 AGGATGATGA TGACCAACCT AGAGGACTCA CCAAAGAACA GATTGACAAC TTGGCAATGA 1741 GAAGTTTTGG TGAAAATGAT GCATTAAAAA CCTGTAGTGT TTGCATTACA GAATATACAG 1801 AAGGCAACAA ACTTCGTAAA CTACCTTGTT CCCATGAGTA CCATGTCCAC TGCATCGATC 1861 GCTGGTTATC TGAGAATTCT ACCTGTCCTA TTTGTCGCAG AGCAGTCTTA GCTTCTGGTA 1921 ACAGAGAAAG TGTTGTGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1981 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2041 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACCATCA TGATTGCCCT 2101 GGGATGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt