Construct: ORF TRCN0000467298
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000614.1_s317c1
- Derived from:
- ccsbBroadEn_00689
- DNA Barcode:
- TGACGTCCGTCCCAGACCGTCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRB7 (2886)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467298
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2886 | GRB7 | growth factor receptor boun... | NM_001030002.2 | 100% | 100% | |
| 2 | human | 2886 | GRB7 | growth factor receptor boun... | NM_001242443.1 | 100% | 100% | |
| 3 | human | 2886 | GRB7 | growth factor receptor boun... | NM_005310.5 | 100% | 100% | |
| 4 | human | 2886 | GRB7 | growth factor receptor boun... | NM_001242442.1 | 95.8% | 95.8% | 1_69del |
| 5 | human | 2886 | GRB7 | growth factor receptor boun... | NM_001330207.2 | 84% | 78.7% | 1269_1270ins88;1341_1342ins167 |
| 6 | human | 2886 | GRB7 | growth factor receptor boun... | XM_024450729.1 | 84% | 78.7% | 1269_1270ins88;1341_1342ins167 |
| 7 | mouse | 14786 | Grb7 | growth factor receptor boun... | NM_010346.2 | 85.2% | 90.1% | (many diffs) |
| 8 | mouse | 14786 | Grb7 | growth factor receptor boun... | XM_006532233.1 | 85.2% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctggatctg tctccacctc atcttagcag ctctccggaa gacctttgcc 121 cagcccctgg gacccctcct gggactcccc ggccccctga tacccctctg cctgaggagg 181 taaagaggtc ccagcctctc ctcatcccaa ccaccggcag gaaacttcga gaggaggaga 241 ggcgtgccac ctccctcccc tctatcccca accccttccc tgagctctgc agtcctccct 301 cacagagccc aattctcggg ggcccctcca gtgcaagggg gctgctcccc cgcgatgcca 361 gccgccccca tgtagtaaag gtgtacagtg aggatggggc ctgcaggtct gtggaggtgg 421 cagcaggtgc cacagctcgc cacgtgtgtg aaatgctggt gcagcgagct cacgccttga 481 gcgacgagac ctgggggctg gtggagtgcc acccccacct agcactggag cggggtttgg 541 aggaccacga gtccgtggtg gaagtgcagg ctgcctggcc cgtgggcgga gatagccgct 601 tcgtcttccg gaaaaacttc gccaagtacg aactgttcaa gagctcccca cactccctgt 661 tcccagaaaa aatggtctcc agctgtctcg atgcacacac tggtatatcc catgaagacc 721 tcatccagaa cttcctgaat gctggcagct ttcctgagat ccagggcttt ctgcagctgc 781 ggggttcagg acggaagctt tggaaacgct ttttctgctt cttgcgccga tctggcctct 841 attactccac caagggcacc tctaaggatc cgaggcacct gcagtacgtg gcagatgtga 901 acgagtccaa cgtgtacgtg gtgacgcagg gccgcaagct ctacgggatg cccactgact 961 tcggtttctg tgtcaagccc aacaagcttc gaaatggcca caaggggctt cggatcttct 1021 gcagtgaaga tgagcagagc cgcacctgct ggctggctgc cttccgcctc ttcaagtacg 1081 gggtgcagct gtacaagaat taccagcagg cacagtctcg ccatctgcat ccatcttgtt 1141 tgggctcccc acccttgaga agtgcctcag ataataccct ggtggccatg gacttctctg 1201 gccatgctgg gcgtgtcatt gagaaccccc gggaggctct gagtgtggcc ctggaggagg 1261 cccaggcctg gaggaagaag acaaaccacc gcctcagcct gcccatgcca gccTCCGGCA 1321 CGAGCCTCAG TGCAGCCATC CACCGCACCC AACTCTGGTT CCACGGGCGC ATTTCCCGTG 1381 AGGAGAGCCA GCGGCTTATT GGACAGCAGG GCTTGGTAGA CGGCCTGTTC CTGGTCCGGG 1441 AGAGTCAGCG GAACCCCCAG GGCTTTGTCC TCTCTTTGTG CCACCTGCAG AAAGTGAAGC 1501 ATTATCTCAT CCTGCCGAGC GAGGAGGAGG GCCGCCTGTA CTTCAGCATG GATGATGGCC 1561 AGACCCGCTT CACTGACCTG CTGCAGCTCG TGGAGTTCCA CCAGCTGAAC CGCGGCATCC 1621 TGCCGTGCTT GCTGCGCCAT TGCTGCACGC GGGTGGCCCT CTGCCCAACT TTCTTGTACA 1681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1741 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1801 AGGACGATGA CGTCCGTCCC AGACCGTCTG CACGCGTTAA GTCgacaatc aacctctgga 1861 ttacaaaatt tgtgaaagat t