Construct: ORF TRCN0000467308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011687.1_s317c1
- Derived from:
- ccsbBroadEn_04213
- DNA Barcode:
- TAGACCTGTTCCCTTGTCGCGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- REEP4 (80346)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467308
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80346 | REEP4 | receptor accessory protein 4 | NM_025232.4 | 100% | 100% | |
| 2 | human | 80346 | REEP4 | receptor accessory protein 4 | NM_001316964.2 | 67.8% | 75% | 552_553ins155;617_753del |
| 3 | human | 80346 | REEP4 | receptor accessory protein 4 | NM_001316965.2 | 67.3% | 57.4% | 416_417ins136;519_520ins116 |
| 4 | mouse | 72549 | Reep4 | receptor accessory protein 4 | NM_180588.2 | 85.2% | 88.3% | (many diffs) |
| 5 | mouse | 72549 | Reep4 | receptor accessory protein 4 | XM_006519598.3 | 74.7% | 77.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gtcctggatg atctgtcgcc tggtggtgct ggtgtttggg atgctgtgtc 121 cagcttatgc ttcctataag gctgtgaaga ccaagaacat tcgtgaatat gtgcggtgga 181 tgatgtactg gattgttttt gcactcttca tggcagcaga gatcgttaca gacattttta 241 tctcctggtt ccctttctac tatgagatca agatggcctt cgtgctgtgg ctgctctcac 301 cctacaccaa gggcgccagc ctgctttacc gcaagtttgt ccacccgtcc ctgtcccgcc 361 atgagaagga gatcgacgcg tacatcgtgc aggccaagga gcgcagctac gagaccgtgc 421 tcagcttcgg gaagcggggc ctcaacattg ccgccTCCGC TGCTGTGCAG GCTGCCACCA 481 AGAGTCAGGG GGCGCTGGCC GGCAGGCTGC GGAGCTTCTC CATGCAGGAC CTGCGCTCCA 541 TCTCTGACGC ACCTGCCCCT GCCTACCATG ACCCCCTCTA CCTGGAGGAC CAGGTGTCCC 601 ACCGGAGGCC ACCCATTGGG TACCGGGCCG GGGGCCTGCA GGACAGCGAC ACCGAGGATG 661 AGTGTTGGTC AGATACTGAG GCAGTCCCCC GGGCGCCAGC CCGGCCCCGA GAGAAGCCCC 721 TAATCCGCAG CCAGAGCCTG CGTGTGGTCA AGAGGAAGCC ACCGGTGCGG GAGGGCACCT 781 CGCGCTCCCT GAAGGTTCGG ACGAGGAAAA AGACTGTGCC CTCAGACGTG GACAGCTACC 841 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 901 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 961 ATATATCTTG TGGAAAGGAC GATAGACCTG TTCCCTTGTC GCGGCAACGC GTTAAGTCga 1021 caatcaacct ctggattaca aaatttgtga aagatt