Construct: ORF TRCN0000467310
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007178.1_s317c1
- Derived from:
- ccsbBroadEn_06529
- DNA Barcode:
- ACTAATTTTCTAGTCCGAAAAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMNB1 (4001)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467310
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4001 | LMNB1 | lamin B1 | NM_005573.4 | 99.9% | 99.8% | 1144G>C |
| 2 | human | 4001 | LMNB1 | lamin B1 | NM_001198557.2 | 64.1% | 63.9% | 0_1ins630;514G>C |
| 3 | human | 4001 | LMNB1 | lamin B1 | NR_134488.1 | 50.2% | (many diffs) | |
| 4 | mouse | 16906 | Lmnb1 | lamin B1 | NM_010721.2 | 88.1% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1824
- ORF length:
- 1758
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gactgcgacc cccgtgccgc cgcggatggg cagccgcgct ggcggcccca 121 ccacgccgct gagccccacg cgcctgtcgc ggctccagga gaaggaggag ctgcgcgagc 181 tcaatgaccg gctggcggtg tacatcgaca aggtgcgcag cctggagacg gagaacagcg 241 cgctgcagct gcaggtgacg gagcgcgagg aggtgcgcgg ccgtgagctc accggcctca 301 aggcgctcta cgagaccgag ctggccgacg cgcgacgcgc gctcgacgac acggcccgcg 361 agcgcgccaa gctgcagatc gagctgggca agtgcaaggc ggaacacgac cagctgctcc 421 tcaactatgc taagaaggaa tctgatctta atggcgccca gatcaagctt cgagaatatg 481 aagcagcact gaattcgaaa gatgcagctc ttgctactgc acttggtgac aaaaaaagtt 541 tagagggaga tttggaggat ctgaaggatc agattgccca gttggaagcc tccttagctg 601 cagccaaaaa acagttagca gatgaaactt tacttaaagt agatttggag aatcgttgtc 661 agagccttac tgaggacttg gagtttcgca aaagcatgta tgaagaggag attaacgaga 721 ccagaaggaa gcatgaaacg cgcttggtag aggtggattc tgggcgtcaa attgagtatg 781 agtacaagct ggcgcaagcc cttcatgaga tgagagagca acatgatgcc caagtgaggc 841 tgtataagga ggagctggag cagacttacc atgccaaact tgagaatgcc agactgtcat 901 cagagatgaa tacttctact gtcaacagtg ccagggaaga actgatggaa agccgcatga 961 gaattgagag cctttcatcc cagctttcta atctacagaa agagtctaga gcatgtttgg 1021 aaaggattca agaattagag gacttgcttg ctaaagaaaa agacaactct cgtcgcatgc 1081 tgacagacaa agagagagag atggcggaaa taagggatca aatgcagcaa cagctgaatg 1141 actatgaaca gcttcttgat gtaaagttag ccctggacat ggaaatcagt gcttacagga 1201 aactcttaca aggcgaagaa gagaggttga agctgtctcc aagcccttct tcccgtgtga 1261 cagtatcccg agcatcctca agtcgtagtg tacgtacaac tagaggaaag cggaagaggg 1321 ttgatgtgga agaatcagag gcgagtagta gtgttagcat ctctcattcc gcctcagcca 1381 ctggaaatgt ttgcatcgaa gaaattgatg ttgatgggaa atttaTCCGC TTGAAGAACA 1441 CTTCTGAACA GGATCAACCA ATGGGAGGCT GGGAGATGAT CAGAAAAATT GGAGACACAT 1501 CAGTCAGTTA TAAATATACC TCAAGATATG TGCTGAAGGC AGGCCAGACT GTTACAATTT 1561 GGGCTGCAAA CGCTGGTGTC ACAGCCAGCC CCCCAACTGA CCTCATCTGG AAGAACCAGA 1621 ACTCGTGGGG CACTGGCGAA GATGTGAAGG TTATATTGAA AAATTCTCAG GGAGAGGAGG 1681 TTGCTCAAAG AAGTACAGTC TTTAAAACAA CCATACCTGA AGAAGAGGAG GAGGAGGAAG 1741 AAGCAGCTGG AGTGGTTGTT GAGGAAGAAC TTTTCCACCA GCAGGGAACC CCAAGAGCAT 1801 CCAATAGAAG CTGTGCAATT ATGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1861 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1921 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CTAATTTTCT 1981 AGTCCGAAAA GTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2041 att