Construct: ORF TRCN0000467331
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011072.1_s317c1
- Derived from:
- ccsbBroadEn_12588
- DNA Barcode:
- ATTAGTGATGACCTCCACCAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C3orf52 (79669)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467331
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79669 | C3orf52 | chromosome 3 open reading f... | NM_024616.2 | 69.1% | 69.1% | 1_201del |
| 2 | human | 79669 | C3orf52 | chromosome 3 open reading f... | XR_924171.3 | 4.8% | 1_260del;489G>A;711_9214del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 516
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat catcaccTCC ATTTTCCTAG GTGTCATTAC AGTGATCATC ATAGGCTTAT 121 GTCTTGCTGC AGTAACTTAT GTTGATGAAG ATGAAAATGA AATACTTGAA TTATCATCAA 181 ACAAAACATT CTTCATCATG CTGAAGATTC CAGAGGAGTG TGTTGCTGAA GAGGAATTGC 241 CTCACCTGCT CACCGAAAGG CTCACAGATG TGTACAGTAC ATCGCCCTCT CTGAGTCGTT 301 ATTTTACTTC AGTTGAAATA GTGGACTTCA GTGGTGAAAA TGCCACAGTA ACGTATGACC 361 TGCAATTTGG GGTTCCATCA GATGATGAAA ATTTTATGAA GTATATGATG AGTGAGGAGT 421 TGGTGCTGGG CATTTTGCTA CAGGATTTCC GTGATCAGAA TATACCTGGT TGTGAGAGTC 481 TGGGGCTTGA TCCAACATCC CTCTTGCTCT ATGAATGCCC AACTTTCTTG TACAAAGTGG 541 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 601 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 661 AATTAGTGAT GACCTCCACC AATATACGCG TTAAGTCgac aatcaacctc tggattacaa 721 aatttgtgaa agatt