Construct: ORF TRCN0000467334
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007301.1_s317c1
- Derived from:
- ccsbBroadEn_14161
- DNA Barcode:
- TCCACTCCTTAATCAGCTTTAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPEAR (54084)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467334
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54084 | TSPEAR | thrombospondin type laminin... | NM_001272037.2 | 87.6% | 85.1% | (many diffs) |
| 2 | human | 54084 | TSPEAR | thrombospondin type laminin... | NM_144991.3 | 78.7% | 76.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1656
- ORF length:
- 1590
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cttcccagca tccaggattt tctcccagtg tgacctcttc cctgaagaat 121 tttccatcgt cgtaactttg agagttccca atcttccacc caagaggaac gagtacctgc 181 tgacggtggt ggcagaggag agcgacctgc tgctgctcgg cctgcggttg tcacctgccc 241 agctgcactt cctgttcctt cgcgaggaca cggccggcgc ctggcagacc cgagtgtcct 301 tccgcagccc ggccctggtg gatggccgct ggcacacact ggtcctggct gtgtccgcag 361 gcgtcttctc cctcaccacg gactgcggcc tcccggtgga cataatggcc gatgtgccct 421 tcccagccac cctgtcagtg aaaggagctc gattcttcgt cggcagccgg aggagagcca 481 aaggcctgtt catgggactg gtgaggcaac tggtcctgct gccgggctca gacgccaccc 541 caaggctgtg tcccagcagg aacgccccgc tggcggtgct gtccatccca cgggtcctgc 601 aggctctcac ngggaagcca gaagataacg aggtgctaaa atatccctat gaaaccaaca 661 ttcgagtgac gctgggaccc cagccaccgt gtaccgaggt ggaagacgcc cagttctggt 721 ttgatgccag ccggaagggc ctgtatctgt gtgttggcaa cgagtgggtc tccgtgttag 781 cagccaaaga aagactggac tacgtggagg agcatcagaa cttgtccacc aactcagaga 841 ccctgggcat tgaggtgttc cgcatccctc aggtggggct ctttgtggcc acagccaatc 901 gcaaagccac atccgccgtc tacaagtgga ccgaagagaa gttcgtctca tatcagaaca 961 tccccacgca ccaagcacag gcctggaggc atttcaccat cgggaaaaag atcttcctgg 1021 cagtggctaa ttttgaacca gatgagaagg gtcaggagtt ctctgtcatt tacaaatgga 1081 gccacagaaa gctgaagttt accccatatc agagcattgc cacacacagc gcccgagact 1141 gggaggcctt cgaggtggat ggggagcact tcctggcggt ggccaaccac cgggaaggcg 1201 acaaccacaa catcgacagt gtcatctaca agtggaaccc ggcaacccgg ctcttcgagg 1261 ccaaccagac catcgccacc TCCGGCGCCT ACGACTGGGA GTTCTTCAGT GTGGGGCCCT 1321 ACTCGTTCCT GGTGGTGGCC AACACCTTCA ACGGCACCTC CACCAAGGTG CACTCGCACC 1381 TCTACATCCG ACTCCTGGGC TCCTTCCAGC TCTTCCAGTC CTTCCCGACG TTCGGTGCTG 1441 CAGACTGGGA GGTCTTCCAG ATCGGGGAGA GGATCTTCCT CGCTGTGGCA AACAGTCACA 1501 GCTACGATGT GGAGATGCAA GTCCAGAATG ATTCCTATGT CATCAACTCC GTCATCTACG 1561 AGCTGAACGT GACCGCGCAG GCCTTTGTCA AGTTCCAGGA CATTCTCACC TGCAGTGGAG 1621 GCAGGAGGGG GCTTCATGCC CGTCGTGCGG AGGGATACCC AACTTTCTTG TACAAAGTGG 1681 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1741 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1801 ATCCACTCCT TAATCAGCTT TAACTACGCG TTAAGTCgac aatcaacctc tggattacaa 1861 aatttgtgaa agatt