Construct: ORF TRCN0000467336
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008352.1_s317c1
- Derived from:
- ccsbBroadEn_00590
- DNA Barcode:
- AGGATTCAGGGTCCTTTCTATAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRK (2444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467336
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2444 | FRK | fyn related Src family tyro... | NM_002031.3 | 100% | 100% | |
| 2 | human | 2444 | FRK | fyn related Src family tyro... | XM_005266880.4 | 100% | 100% | |
| 3 | human | 2444 | FRK | fyn related Src family tyro... | XM_005266881.2 | 100% | 100% | |
| 4 | human | 2444 | FRK | fyn related Src family tyro... | XM_005266882.4 | 100% | 100% | |
| 5 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535653.2 | 100% | 100% | |
| 6 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535654.2 | 100% | 100% | |
| 7 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535655.2 | 100% | 100% | |
| 8 | human | 2444 | FRK | fyn related Src family tyro... | XM_017010645.1 | 100% | 100% | |
| 9 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535656.2 | 77.5% | 77.2% | 0_1ins338;2delT;5_6insCC |
| 10 | mouse | 14302 | Frk | fyn-related kinase | NM_001159544.1 | 86% | 88.2% | (many diffs) |
| 11 | mouse | 14302 | Frk | fyn-related kinase | NM_010237.3 | 86% | 88.2% | (many diffs) |
| 12 | mouse | 14302 | Frk | fyn-related kinase | XM_006512534.3 | 86% | 88.2% | (many diffs) |
| 13 | mouse | 14302 | Frk | fyn-related kinase | XM_006512535.3 | 86% | 88.2% | (many diffs) |
| 14 | mouse | 14302 | Frk | fyn-related kinase | XM_006512536.3 | 86% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1581
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caacatctgt cagaggctct gggagtacct agaaccctat ctcccctgtt 121 tgtccacgga ggcagacaag tcaaccgtga ttgaaaatcc aggggccctt tgctctcccc 181 agtcacagag gcatggccac tactttgtgg ctttgtttga ttaccaggct cggactgctg 241 aggacttgag cttccgagca ggtgacaaac ttcaagttct ggacactttg catgagggct 301 ggtggtttgc cagacacttg gagaaaagac gagatggctc cagtcagcaa ctacaaggct 361 atattccttc taactacgtg gctgaggaca gaagcctaca ggcagagccg tggttctttg 421 gagcaatcgg aagatcagat gcagagaaac aactattata ttcagaaaac aagaccggtt 481 cctttctaat cagagaaagt gaaagccaaa aaggagaatt ctctctttca gttttagatg 541 gagcagttgt aaaacactac agaattaaaa gactggatga agggggattt tttctcacgc 601 gaagaagaat cttttcaaca ctgaacgaat ttgtgagcca ctacaccaag acaagtgacg 661 gcctgtgtgt caagctgggg aaaccatgct taaagatcca ggtcccagct ccatttgatt 721 tgtcgtataa aaccgtggac caatgggaga tagaccgcaa ctccatacag cttctgaagc 781 gattgggatc tggtcagttt ggcgaagtat gggaaggtct gtggaacaat accactccag 841 tagcagtgaa aacattaaaa ccaggttcaa tggatccaaa tgacttcctg agggaggcac 901 agataatgaa gaacctaaga catccaaagc ttatccagct ttatgctgtt tgcactttag 961 aagatccaat ttatattatt acagagttga tgagacatgg aagtctgcaa gaatatctcc 1021 aaaatgacac tggatcaaaa atccatctga ctcaacaggt agacatggcg gcacaggttg 1081 cctctggaat ggcctatctg gagtctcgga actacattca cagagatctg gctgccagaa 1141 atgTCCTCGT TGGTGAACAT AATATCTACA AAGTAGCAGA TTTTGGACTT GCCAGAGTTT 1201 TTAAGGTAGA TAATGAAGAC ATCTATGAAT CTAGACACGA AATAAAGCTG CCGGTGAAGT 1261 GGACTGCGCC CGAAGCCATT CGTAGTAATA AATTCAGCAT TAAGTCCGAT GTATGGTCAT 1321 TTGGAATCCT TCTTTATGAA ATCATTACTT ATGGCAAAAT GCCTTACAGT GGTATGACAG 1381 GTGCCCAGGT AATCCAGATG TTGGCTCAAA ACTATAGACT TCCGCAACCA TCCAACTGTC 1441 CACAGCAATT TTACAACATC ATGTTGGAGT GCTGGAATGC AGAGCCTAAG GAACGACCTA 1501 CATTTGAGAC ACTGCGTTGG AAACTTGAAG ACTATTTTGA AACAGACTCT TCATATTCAG 1561 ATGCAAATAA CTTCATAAGA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGGA TTCAGGGTCC 1741 TTTCTATAGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt