Construct: ORF TRCN0000467389
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005260.1_s317c1
- Derived from:
- ccsbBroadEn_05164
- DNA Barcode:
- CAGTCTCATTCTCTGGCACTACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM9B (171483)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467389
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 171483 | FAM9B | family with sequence simila... | NM_205849.3 | 100% | 100% | |
| 2 | human | 171483 | FAM9B | family with sequence simila... | XM_024452346.1 | 100% | 100% | |
| 3 | human | 171483 | FAM9B | family with sequence simila... | XM_024452345.1 | 80.5% | 80.5% | 1_135del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 627
- ORF length:
- 558
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcctgg gggaagaagc atgcaggaaa ggatccagtc cgtgatgaat 121 gtgaggaaag aaaccgtttt acagaaacaa gggaggaaga tgtaactgat gagcatgggg 181 aaagagaacc ttttgcTGAA ACAGATGAAC ACACGGGGGC TAATACCAAG AAGCCAGAAG 241 ATACTGCAGA GGATCTTACT GCAAAAAGAA AAAGGATGAA AATGGATAAA ACTTGCAGCA 301 AAACAAAGAA CAAAAGTAAA CATGCTTTGA GAAAAAAGCA ACTTAAAAGG CAGAAACGTG 361 ATTATATACA TTCTCTGAAG TTGCTAAATG TCCTTGAAGA ATACATCACA GACGAGCAGA 421 AAGAGGAAGA AGAAGAAGAG GGAGAAGAGG AAGAACTAAT TAGAATATTT CAAGAACAAC 481 AGAAGAAGTG GCAACAATAT AGAAGTGTTA GGAGAGAGAG GCTGAAAGAG ATGAAGCTGC 541 TACGTGACCA ATTCGTAAAG GCTCTTGAGG ACTTTGAAGA CCTTTGTGAC AGAGTTTTTT 601 CCGATGAAGA CAGTGAACTT GATAACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACAGTCTCA 781 TTCTCTGGCA CTACGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 841 aagatt