Construct: ORF TRCN0000467397
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004713.1_s317c1
- Derived from:
- ccsbBroadEn_11832
- DNA Barcode:
- TTTCTTTCTTCATTCCGACATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCOLCE2 (26577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467397
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26577 | PCOLCE2 | procollagen C-endopeptidase... | NM_013363.4 | 66.5% | 66.5% | 829_1245del |
| 2 | mouse | 76477 | Pcolce2 | procollagen C-endopeptidase... | NM_029620.2 | 56.7% | 58.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 894
- ORF length:
- 828
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgtcatgag gggcgcgaac gcctgggcgc cactctgcct gctgctggct gccgccaccc 121 agctctcgcg gcagcagtcc ccagagagac ctgttttcac atgtggtggc attcttactg 181 gagagtctgg atttattggc agtgaaggtt ttcctggagt gtaccctcca aatagcaaat 241 gtacttggaa aatcacagtt cccgaaggaa aagtagtcgt tctcaatttc cgattcatag 301 acctcgagag tgacaacctg tgccgctatg actttgtgga tgtgtacaat ggccatgcca 361 atggccagcg cattggccgc ttctgtggca ctttccggcc tggagccctt gtgtccagtg 421 gcaacaagat gatggtgcag atgatttctg atgccaacac agctggcaat ggcttcatgg 481 ccatgttctc cgctgctgaa ccaaacgaaa gaggggatca gtattgtgga ggactccttg 541 acagaccttc cggctctttt aaaaccccca actggccaga ccgggattac cctgcaggag 601 tcacttgtgt gtggcacatt gtagccccaa agaatcagct tatagaatta aagtttgaga 661 agtttgatgt ggagcgagat aactactgcc gatatgatta tgtggctgtg tttaatggcg 721 gggaagtcaa cgatgctaga agaattggaa agtattgtgg tgatagtcca cctgcgccaa 781 ttgtgtctga gagaaatgaa cttcTTATTC AGTTTTTATC AGACTTAAGT TTAACTGCAG 841 ATGGGTTTAT TGGTCACTAC ATATTCAGGC CAAAAAAACT GCCTACAACT ACATACCCAA 901 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 961 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1021 TATCTTGTGG AAAGGACGAT TTCTTTCTTC ATTCCGACAT TTTACGCGTT AAGTCgacaa 1081 tcaacctctg gattacaaaa tttgtgaaag att