Construct: ORF TRCN0000467400
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013835.1_s317c1
- Derived from:
- ccsbBroadEn_02677
- DNA Barcode:
- AACCTTGCGGGTGACGCCCTCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDAP1 (11333)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467400
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11333 | PDAP1 | PDGFA associated protein 1 | NM_014891.7 | 100% | 100% | |
| 2 | mouse | 231887 | Pdap1 | PDGFA associated protein 1 | NM_001033313.3 | 89.6% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc taaaggagga agaaagggag gccacaaagg ccgggcgagg cagtatacaa 121 gccctgagga gatcgacgcg cagctgcagg ctgagaagca gaaggccagg gaagaagagg 181 agcaaaaaga aggtggagat ggggctgcag gtgaccccaa aaaggagaag aaatctctag 241 actcagatga gagtgaggat gaagaagatg actaccagca aaagcgcaaa ggcgttgaag 301 ggctcatcga catcgagaac cccaaccggg tggcacagac aaccaaaaag gtcacacaac 361 tggatctgga cgggccaaag gagctttcga ggagagaacg agaagagatt gagaagcaga 421 aggcaaaaga gcgttacatg aaaatgcact tggccgggaa gacagagcaa gccaaggctg 481 acctggcccg gctggccatc atccggaaac agcgggagga ggctgcccgg aagaaggaag 541 aggaaaggaa agcaaaagac gatgccacat tGTCAGGAAA ACGAATGCAG TCACTCTCCC 601 TGAATAAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACCTT GCGGGTGACG CCCTCTAAAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt