Construct: ORF TRCN0000467417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000597.1_s317c1
- Derived from:
- ccsbBroadEn_07557
- DNA Barcode:
- CGTAGTAACGCTTTCGGATACGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAMPT (10135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10135 | NAMPT | nicotinamide phosphoribosyl... | NM_005746.3 | 99.9% | 100% | 903A>G |
| 2 | mouse | 59027 | Nampt | nicotinamide phosphoribosyl... | NM_021524.2 | 91.4% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1539
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tcctgcggca gaagccgagt tcaacatcct cctggccacc gactcctaca 121 aggttactca ctataaacaa tatccaccca acacaagcaa agtttattcc tactttgaat 181 gccgtgaaaa gaagacagaa aactccaaat taaggaaggt gaaatatgag gaaacagtat 241 tttatgggtt gcagtacatt cttaataagt acttaaaagg taaagtagta accaaagaga 301 aaatccagga agccaaagat gtctacaaag aacatttcca agatgatgtc tttaatgaaa 361 agggatggaa ctacattctt gagaagtatg atgggcatct tccaatagaa ataaaagctg 421 ttcctgaggg ctttgtcatt cccagaggaa atgttctctt cacggtggaa aacacagatc 481 cagagtgtta ctggcttaca aattggattg agactattct tgttcagtcc tggtatccaa 541 tcacagtggc cacaaattct agagagcaga agaaaatatt ggccaaatat ttgttagaaa 601 cttctggtaa cttagatggt ctggaataca agttacatga ttttggctac agaggagtct 661 cttcccaaga gactgctggc ataggagcat ctgctcactt ggttaacttc aaaggaacag 721 atacagtagc aggacttgct ctaattaaaa aatattatgg aacgaaagat cctgttccag 781 gctattctgt tccagcagca gaacacagta ccataacagc ttgggggaaa gaccatgaaa 841 aagatgcttt tgaacatatt gtaacacagt tttcatcagt gcctgtatct gtggtcagcg 901 atagctatga catttataat gcgtgtgaga aaatatgggg tgaagatcta agacatttaa 961 tagtatcgag aagtacacag gcaccactaa taatcagacc tgattctgga aaccctcttg 1021 acactgtgtt aaaggttttg gagattttag gtaagaagtt tcctgttact gagaactcaa 1081 agggttacaa gttgctgcca ccttatctta gagttattca aggggatgga gtagatatta 1141 ataCCTTACA AGAGATTGTA GAAGGCATGA AACAAAAAAT GTGGAGTATT GAAAATATTG 1201 CCTTCGGTTC TGGTGGAGGT TTGCTACAGA AGTTGACAAG AGATCTCTTG AATTGTTCCT 1261 TCAAGTGTAG CTATGTTGTA ACTAATGGCC TTGGGATTAA CGTCTTCAAG GACCCAGTTG 1321 CTGATCCCAA CAAAAGGTCC AAAAAGGGCC GATTATCTTT ACATAGGACG CCAGCAGGGA 1381 ATTTTGTTAC ACTGGAGGAA GGAAAAGGAG ACCTTGAGGA ATATGGTCAG GATCTTCTCC 1441 ATACTGTCTT CAAGAATGGC AAGGTGACAA AAAGCTATTC ATTTGATGAA ATAAGAAAAA 1501 ATGCACAGCT GAATATTGAA CTGGAAGCAG CACATCATTA CCCAACTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGACGTAGT AACGCTTTCG GATACGATAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt