Construct: ORF TRCN0000467422
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009061.1_s317c1
- Derived from:
- ccsbBroadEn_02585
- DNA Barcode:
- TCCTTTCTTTCACTGACCGCTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COPS6 (10980)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467422
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10980 | COPS6 | COP9 signalosome subunit 6 | NM_006833.5 | 100% | 100% | |
| 2 | mouse | 26893 | Cops6 | COP9 signalosome subunit 6 | NM_012002.3 | 92.4% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcggcg gcggcggctg cagctacgaa cgggaccgga ggaagcagcg 121 ggatggaggt ggatgcagca gtagtcccca gcgtgatggc ctgcggagtg actgggagtg 181 tttccgtcgc tctccatccc cttgtcattc tcaacatctc agaccactgg atccgcatgc 241 gctcccagga ggggcggcct gtgcaggtga ttggggctct gattggcaag caggagggcc 301 gaaatatcga ggtgatgaac tcctttgagc tgctgtccca caccgtggaa gagaagatta 361 tcattgacaa ggaatattat tacaccaagg aggagcagtt taaacaggtg ttcaaggagc 421 tggagtttct gggttggtat accacagggg ggccacctga cccctcggac atccacgtcc 481 ataagcaggt gtgtgagatc atcgagagcc ccctctttct gaagttgaac cctatgacca 541 agcacacaga tcttcctgtc agcgtttttg agtctgtcat tgatataatc aatggagagg 601 ccacaatgct gtttgctgag ctgacctaca ctctggCCAC AGAGGAAGCG GAACGCATTG 661 GTGTAGACCA CGTAGCCCGA ATGACAGCAA CAGGCAGTGG AGAGAACTCC ACTGTGGCTG 721 AACACCTGAT AGCACAGCAC AGCGCCATCA AGATGCTGCA CAGCCGCGTC AAGCTCATCT 781 TGGAGTACGT CAAGGCCTCT GAAGCGGGAG AGGTCCCCTT TAATCATGAG ATCCTGCGGG 841 AGGCCTATGC TCTGTGTCAC TGTCTCCCGG TGCTCAGCAC AGACAAGTTC AAGACAGATT 901 TTTATGATCA ATGCAACGAC GTGGGGCTCA TGGCCTACCT CGGCACCATC ACCAAAACGT 961 GCAACACCAT GAACCAGTTT GTGAACAAGT TCAATGTCCT CTACGACCGA CAAGGCATCG 1021 GCAGGAGAAT GCGCGGGCTC TTTTTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCCTTTCT 1201 TTCACTGACC GCTGCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt