Construct: ORF TRCN0000467425
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010539.1_s317c1
- Derived from:
- ccsbBroadEn_11447
- DNA Barcode:
- CGCATGCCTGCGATCATAACATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH2B3 (10019)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467425
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100289019 | SLC25A25-AS1 | SLC25A25 antisense RNA 1 | NR_033374.1 | 3.4% | (many diffs) | |
| 2 | human | 10019 | SH2B3 | SH2B adaptor protein 3 | XR_002957278.1 | 2.7% | 1_3974del;4128_5649del | |
| 3 | human | 10019 | SH2B3 | SH2B adaptor protein 3 | XR_001748536.1 | 2.6% | 1_4004del;4158_5679del | |
| 4 | human | 10019 | SH2B3 | SH2B adaptor protein 3 | XR_001748535.1 | 2.6% | 1_4098del;4252_5773del | |
| 5 | human | 3824 | KLRD1 | killer cell lectin like rec... | NR_147040.2 | .8% | (many diffs) | |
| 6 | human | 3824 | KLRD1 | killer cell lectin like rec... | NR_147039.2 | .8% | (many diffs) | |
| 7 | human | 3824 | KLRD1 | killer cell lectin like rec... | NR_147038.2 | .8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 183
- ORF end:
- 336
- ORF length:
- 153
- Sequence:
-
1 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT 61 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT 121 TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG 181 GCATGGAGTT TCTCTTGTCA ACCGGGCTGG AGTGCAGTGG TGCAATCTTG GCTCACTGCA 241 ACCTCCACCT TCCTGGTTCA AGCGATTCTG CCTCGACCTC TCAAGTAGCT GGGATTACAA 301 GCACCAGCCA CCATGCCTGG CTAATTTTGT ATTTTTACCC AACTTTCTTG TACAAAGTGG 361 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 421 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 481 ACGCATGCCT GCGATCATAA CATCAACGCG TTAAGTCgac aatcaacctc tggattacaa 541 aatttgtgaa agatt