Construct: ORF TRCN0000467426
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014016.1_s317c1
- Derived from:
- ccsbBroadEn_03482
- DNA Barcode:
- TACATCCCGGTGGAATTAGATAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIME1 (54923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467426
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54923 | LIME1 | Lck interacting transmembra... | NM_017806.4 | 100% | 100% | |
| 2 | human | 54923 | LIME1 | Lck interacting transmembra... | NM_001305654.1 | 59.6% | 30.4% | 179_180ins35;528_529ins322 |
| 3 | human | 54923 | LIME1 | Lck interacting transmembra... | NM_001305655.2 | 59.6% | 30.4% | 179_180ins35;528_529ins322 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 951
- ORF length:
- 885
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctgccagtg tcctgggccc ctcctgccct ctgggttcta gggtgctgcg 121 ccctgctcct ctcgctgtgg gcgctgtgca cagcctgccg caggcccgag gacgctgtag 181 cccccaggaa gagggcgcgg aggcagcggg cgaggctgca gggcagtgcg acggcggcgg 241 aagcgtccct actgaggcgg acccacctct gctccctcag caagtcggac accagactgc 301 acgagctgca ccggggcccg cgcagcagca gggccctgcg gcctgccagc atggatctcc 361 tgcgcccaca ctggctggag gtgtccaggg acatcaccgg accgcaggca gccccctctg 421 ccttcccaca ccaggagctg ccccgggctc tgccggcagc tgcagccacc gcagggtgcg 481 ctggcctcga ggccacctat tccaacgtgg ggctggcggc ccttcccggg gtcagcctgg 541 cggccagccc tgtggtggcc gagtatgccc gcgtccagaa gcgcaaaggg acccatcgca 601 gtccccaaga gccacagcag gGGAAGACTG AGGTGACCCC GGCCGCTCAG GTGGACGTCC 661 TGTACTCCAG GGTCTGCAAG CCTAAAAGGA GGGACCCAGG ACCCACCACA GACCCGCTGG 721 ACCCCAAGGG CCAGGGAGCG ATTCTGGCCC TGGCGGGTGA CCTGGCCTAC CAGACCCTCC 781 CGCTCAGGGC CCTGGATGTG GACAGCGGCC CCCTGGAAAA CGTGTATGAG AGCATCCGGG 841 AGCTGGGGGA CCCTGCTGGC AGGAGCAGCA CGTGCGGGGC TGGGACGCCC CCTGCTTCCA 901 GCTGCCCCAG CCTAGGGAGG GGCTGGAGAC CCCTCCCTGC CTCCCTGCCC TGCCCAACTT 961 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1021 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1081 CTTGTGGAAA GGACGATACA TCCCGGTGGA ATTAGATAGG ACGCGTTAAG TCgacaatca 1141 acctctggat tacaaaattt gtgaaagatt