Construct: ORF TRCN0000467429
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011590.1_s317c1
- Derived from:
- ccsbBroadEn_02024
- DNA Barcode:
- AATGTCTTGTTCAGAGGGACGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGF17 (8822)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467429
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8822 | FGF17 | fibroblast growth factor 17 | NM_003867.4 | 100% | 100% | |
| 2 | human | 8822 | FGF17 | fibroblast growth factor 17 | NM_001304478.1 | 94.9% | 94.9% | 69_70ins33 |
| 3 | human | 8822 | FGF17 | fibroblast growth factor 17 | XM_011544683.1 | 90.7% | 88.7% | (many diffs) |
| 4 | human | 8822 | FGF17 | fibroblast growth factor 17 | XM_011544684.2 | 90.7% | 88.7% | (many diffs) |
| 5 | human | 8822 | FGF17 | fibroblast growth factor 17 | XM_011544685.1 | 86% | 84% | (many diffs) |
| 6 | human | 8822 | FGF17 | fibroblast growth factor 17 | XM_005273675.1 | 85.8% | 77.6% | (many diffs) |
| 7 | mouse | 14171 | Fgf17 | fibroblast growth factor 17 | NM_008004.4 | 91.5% | 98.6% | (many diffs) |
| 8 | mouse | 14171 | Fgf17 | fibroblast growth factor 17 | XM_006518551.3 | 91.5% | 98.6% | (many diffs) |
| 9 | mouse | 14171 | Fgf17 | fibroblast growth factor 17 | XM_011244953.2 | 91.5% | 98.6% | (many diffs) |
| 10 | mouse | 14171 | Fgf17 | fibroblast growth factor 17 | XM_006518552.3 | 86.4% | 93.5% | (many diffs) |
| 11 | mouse | 14171 | Fgf17 | fibroblast growth factor 17 | XM_011244954.1 | 73.3% | 79.1% | (many diffs) |
| 12 | mouse | 14171 | Fgf17 | fibroblast growth factor 17 | XM_017315846.1 | 69.2% | 68.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 717
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagccgcc cgcctgctgc ccaacctcac tctgtgctta cagctgctga 121 ttctctgctg tcaaactcag ggggagaatc acccgtctcc taattttaac cagtacgtga 181 gggaccaggg cgccatgacc gaccagctga gcaggcggca gatccgcgag taccaactct 241 acagcaggac cagtggcaag cacgtgcagg tcaccgggcg tcgcatctcc gccaccgccg 301 aggacggcaa caagtttgcc aagctcatag tggagacgga cacgtttggc agccgggttc 361 gcaTCAAAGG GGCTGAGAGT GAGAAGTACA TCTGTATGAA CAAGAGGGGC AAGCTCATCG 421 GGAAGCCCAG CGGGAAGAGC AAAGACTGCG TGTTCACGGA GATCGTGCTG GAGAACAACT 481 ATACGGCCTT CCAGAACGCC CGGCACGAGG GCTGGTTCAT GGCCTTCACG CGGCAGGGGC 541 GGCCCCGCCA GGCTTCCCGC AGCCGCCAGA ACCAGCGCGA GGCCCACTTC ATCAAGCGCC 601 TCTACCAAGG CCAGCTGCCC TTCCCCAACC ACGCCGAGAA GCAGAAGCAG TTCGAGTTTG 661 TGGGCTCCGC CCCCACCCGC CGGACCAAGC GCACACGGCG GCCCCAGCCC CTCACGTTGC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GAAATGTCTT GTTCAGAGGG ACGTGCACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt