Construct: ORF TRCN0000467432
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010180.1_s317c1
- Derived from:
- ccsbBroadEn_06567
- DNA Barcode:
- TCCCGCCCTTTGAACTGACGTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCAM (4162)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467432
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4162 | MCAM | melanoma cell adhesion mole... | NM_006500.3 | 99.9% | 100% | 1800A>G |
| 2 | human | 4162 | MCAM | melanoma cell adhesion mole... | XM_017017759.2 | 96.6% | 96.7% | 1485_1486ins63;1737A>G |
| 3 | human | 4162 | MCAM | melanoma cell adhesion mole... | XM_017017760.2 | 94.9% | 95% | 1548_1549ins96;1704A>G |
| 4 | human | 4162 | MCAM | melanoma cell adhesion mole... | XM_017017761.2 | 93.4% | 92.5% | 1793_1794ins118;1812_1813insTGAGGCAT |
| 5 | human | 4162 | MCAM | melanoma cell adhesion mole... | XM_017017762.2 | 90.2% | 89.3% | 1485_1486ins63;1730_1731ins118;1749_1750insTGAGGCAT |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2004
- ORF length:
- 1938
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcttcccagg ctggtctgcg ccttcttgct cgccgcctgc tgctgctgtc 121 ctcgcgtcgc gggtgtgccc ggagaggctg agcagcctgc gcctgagctg gtggaggtgg 181 aagtgggcag cacagccctt ctgaagtgcg gcctctccca gtcccaaggc aacctcagcc 241 atgtcgactg gttttctgtc cacaaggaga agcggacgct catcttccgt gtgcgccagg 301 gccagggcca gagcgaacct ggggagtacg agcagcggct cagcctccag gacagagggg 361 ctactctggc cctgactcaa gtcacccccc aagacgagcg catcttcttg tgccagggca 421 agcgccctcg gtcccaggag taccgcatcc agctccgcgt ctacaaagct ccggaggagc 481 caaacatcca ggtcaacccc ctgggcatcc ctgtgaacag taaggagcct gaggaggtcg 541 ctacctgtgt agggaggaac gggtacccca ttcctcaagt catctggtac aagaatggcc 601 ggcctctgaa ggaggagaag aaccgggtcc acattcagtc gtcccagact gtggagtcga 661 gtggtttgta caccttgcag agtattctga aggcacagct ggttaaagaa gacaaagatg 721 cccagtttta ctgtgagctc aactaccggc tgcccagtgg gaaccacatg aaggagtcca 781 gggaagtcac cgtccctgtt ttctacccga cagaaaaagt gtggctggaa gtggagcccg 841 tgggaatgct gaaggaaggg gaccgcgtgg aaatcaggtg tttggctgat ggcaaccctc 901 caccacactt cagcatcagc aagcagaacc ccagcaccag ggaggcagag gaagagacaa 961 ccaacgacaa cggggtcctg gtgctggagc ctgcccggaa ggaacacagt gggcgctatg 1021 aatgtcaggg cctggacttg gacaccatga tatcgctgct gagtgaacca caggaactac 1081 tggtgaacta tgtgtctgac gtccgagtga gtcccgcagc ccctgagaga caggaaggca 1141 gcagcctcac cctgacctgt gaggcagaga gtagccagga cctcgagttc cagtggctga 1201 gagaagagac aggccaggtg ctggaaaggg ggcctgtgct tcagttgcat gacctgaaac 1261 gggaggcagg aggcggctat cgctgcgtgg cgtctgtgcc cagcataccc ggcctgaacc 1321 gcacacagct ggtcaacgtg gccatttttg gccccccttg gatggcattc aaggagagga 1381 aggtgtgggt gaaagagaat atggtgttga atctgtcttg tgaagcgtca gggcaccccc 1441 ggcccaccat ctcctggaac gtcaacggca cggcaagtga acaagaccaa gatccacagc 1501 gagtcctgag caccctgaat gtcctcgtga ccccggagct gttggagaca ggtgttgaat 1561 gcacggcctc caacgacctg ggcaaaaaca ccagcatcct cttcctggag ctggtcaatt 1621 taaccaccct cacaccagac tccaacacaa ccactggcct cagcacttcc actgccagtc 1681 cTCATACCAG AGCCAACAGC ACCTCCACAG AGAGAAAGCT GCCGGAGCCG GAGAGCCGGG 1741 GCGTGGTCAT CGTGGCTGTG ATTGTGTGCA TCCTGGTCCT GGCGGTGCTG GGCGCTGTCC 1801 TCTATTTCCT CTATAAGAAG GGCAAGCTGC CGTGCAGGCG CTCAGGGAAG CAGGAGATCA 1861 CGCTGCCCCC GTCTCGTAAG AGCGAACTTG TAGTTGAAGT TAAGTCAGAT AAGCTCCCAG 1921 AAGAGATGGG CCTCCTGCAG GGCAGCAGCG GTGACAAGAG GGCTCCGGGA GACCAGGGAG 1981 AGAAATACAT CGATCTGAGG CATTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2041 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2101 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CCCGCCCTTT 2161 GAACTGACGT CCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2221 att