Construct: ORF TRCN0000467432

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010180.1_s317c1
Derived from:
ccsbBroadEn_06567
DNA Barcode:
TCCCGCCCTTTGAACTGACGTCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCAM (4162)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467432
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4162 MCAM melanoma cell adhesion mole... NM_006500.3 99.9% 100% 1800A>G
2 human 4162 MCAM melanoma cell adhesion mole... XM_017017759.2 96.6% 96.7% 1485_1486ins63;1737A>G
3 human 4162 MCAM melanoma cell adhesion mole... XM_017017760.2 94.9% 95% 1548_1549ins96;1704A>G
4 human 4162 MCAM melanoma cell adhesion mole... XM_017017761.2 93.4% 92.5% 1793_1794ins118;1812_1813insTGAGGCAT
5 human 4162 MCAM melanoma cell adhesion mole... XM_017017762.2 90.2% 89.3% 1485_1486ins63;1730_1731ins118;1749_1750insTGAGGCAT
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2004
ORF length:
1938
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gcttcccagg ctggtctgcg ccttcttgct cgccgcctgc tgctgctgtc
121ctcgcgtcgc gggtgtgccc ggagaggctg agcagcctgc gcctgagctg gtggaggtgg
181aagtgggcag cacagccctt ctgaagtgcg gcctctccca gtcccaaggc aacctcagcc
241atgtcgactg gttttctgtc cacaaggaga agcggacgct catcttccgt gtgcgccagg
301gccagggcca gagcgaacct ggggagtacg agcagcggct cagcctccag gacagagggg
361ctactctggc cctgactcaa gtcacccccc aagacgagcg catcttcttg tgccagggca
421agcgccctcg gtcccaggag taccgcatcc agctccgcgt ctacaaagct ccggaggagc
481caaacatcca ggtcaacccc ctgggcatcc ctgtgaacag taaggagcct gaggaggtcg
541ctacctgtgt agggaggaac gggtacccca ttcctcaagt catctggtac aagaatggcc
601ggcctctgaa ggaggagaag aaccgggtcc acattcagtc gtcccagact gtggagtcga
661gtggtttgta caccttgcag agtattctga aggcacagct ggttaaagaa gacaaagatg
721cccagtttta ctgtgagctc aactaccggc tgcccagtgg gaaccacatg aaggagtcca
781gggaagtcac cgtccctgtt ttctacccga cagaaaaagt gtggctggaa gtggagcccg
841tgggaatgct gaaggaaggg gaccgcgtgg aaatcaggtg tttggctgat ggcaaccctc
901caccacactt cagcatcagc aagcagaacc ccagcaccag ggaggcagag gaagagacaa
961ccaacgacaa cggggtcctg gtgctggagc ctgcccggaa ggaacacagt gggcgctatg
1021aatgtcaggg cctggacttg gacaccatga tatcgctgct gagtgaacca caggaactac
1081tggtgaacta tgtgtctgac gtccgagtga gtcccgcagc ccctgagaga caggaaggca
1141gcagcctcac cctgacctgt gaggcagaga gtagccagga cctcgagttc cagtggctga
1201gagaagagac aggccaggtg ctggaaaggg ggcctgtgct tcagttgcat gacctgaaac
1261gggaggcagg aggcggctat cgctgcgtgg cgtctgtgcc cagcataccc ggcctgaacc
1321gcacacagct ggtcaacgtg gccatttttg gccccccttg gatggcattc aaggagagga
1381aggtgtgggt gaaagagaat atggtgttga atctgtcttg tgaagcgtca gggcaccccc
1441ggcccaccat ctcctggaac gtcaacggca cggcaagtga acaagaccaa gatccacagc
1501gagtcctgag caccctgaat gtcctcgtga ccccggagct gttggagaca ggtgttgaat
1561gcacggcctc caacgacctg ggcaaaaaca ccagcatcct cttcctggag ctggtcaatt
1621taaccaccct cacaccagac tccaacacaa ccactggcct cagcacttcc actgccagtc
1681cTCATACCAG AGCCAACAGC ACCTCCACAG AGAGAAAGCT GCCGGAGCCG GAGAGCCGGG
1741GCGTGGTCAT CGTGGCTGTG ATTGTGTGCA TCCTGGTCCT GGCGGTGCTG GGCGCTGTCC
1801TCTATTTCCT CTATAAGAAG GGCAAGCTGC CGTGCAGGCG CTCAGGGAAG CAGGAGATCA
1861CGCTGCCCCC GTCTCGTAAG AGCGAACTTG TAGTTGAAGT TAAGTCAGAT AAGCTCCCAG
1921AAGAGATGGG CCTCCTGCAG GGCAGCAGCG GTGACAAGAG GGCTCCGGGA GACCAGGGAG
1981AGAAATACAT CGATCTGAGG CATTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
2041AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
2101TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CCCGCCCTTT
2161GAACTGACGT CCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2221att

Download FASTA (ORF) (Full)