Construct: ORF TRCN0000467435
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003719.1_s317c1
- Derived from:
- ccsbBroadEn_09725
- DNA Barcode:
- GAACTTAAGGTCCAATAAACACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAAH2 (158584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467435
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | NM_174912.3 | 99.8% | 100% | 822T>C;1404T>C |
| 2 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | NM_001353840.1 | 92.3% | 92.4% | 822T>C;993_994ins120;1284T>C |
| 3 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | XM_017029290.2 | 90.9% | 89.5% | (many diffs) |
| 4 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | NM_001353841.1 | 86.7% | 86.8% | 411_412ins210;612T>C;1194T>C |
| 5 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | XM_005261985.3 | 84.2% | 84.3% | 0_1ins249;573T>C;1155T>C |
| 6 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | XM_011530767.3 | 84.2% | 84.3% | 0_1ins249;573T>C;1155T>C |
| 7 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | XM_017029292.1 | 77.7% | 76.4% | (many diffs) |
| 8 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | NR_148564.2 | 76.7% | (many diffs) | |
| 9 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | NR_148557.2 | 75.7% | (many diffs) | |
| 10 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | XM_017029293.2 | 65.9% | 63.1% | (many diffs) |
| 11 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | XM_005261986.4 | 63.9% | 62.9% | (many diffs) |
| 12 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | XM_017029294.2 | 63.4% | 62.7% | (many diffs) |
| 13 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | XM_005261987.4 | 63.2% | 63.3% | (many diffs) |
| 14 | human | 158584 | FAAH2 | fatty acid amide hydrolase 2 | XR_001755657.1 | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc accttcattt accgcccgca ttcagttgtt cctcttgcgg gcgctaggct 121 ttctcatagg cttagtaggc cgagcagctt tagtcttagg gggtccaaag tttgcctcaa 181 agacccctcg gccggtgact gaaccattgc ttctgctttc ggggatgcag ctggccaagc 241 tgatccgaca gagaaaggtg aaatgtatag atgttgttca ggcttatatc aacagaatca 301 aggacgtgaa cccaatgatc aatggaattg tcaagtacag gtttgaggaa gcgatgaagg 361 aggctcatgc tgtagatcaa aagcttgcag agaagcagga agatgaagcc accctggaaa 421 ataaatggcc cttccttggg gttcctttga cagtcaagga agctttccag ctacaaggaa 481 tgcccaattc ttctggactc atgaaccgtc gtgatgccat tgccaaaaca gatgccactg 541 tggtggcatt actgaaggga gctggtgcca ttcctcttgg cataaccaac tgtagtgagt 601 tgtgtatgtg gtatgaatcc agtaacaaga tctatggccg atcaaacaac ccatatgatt 661 tacagcatat tgtaggtgga agttctggtg gtgagggctg cacactggca gctgcctgct 721 cagttattgg tgtgggctct gatattggtg gtagcattcg aatgcctgct ttcttcaatg 781 gtatatttgg acacaagcct tctccaggtg tggttcccaa caaaggtcag tttcccttgg 841 ctgtgggagc ccaggagttg tttctgtgca ctggtcctat gtgccgctat gctgaagacc 901 tggcccccat gttgaaggtc atggcaggac ctgggatcaa aaggttaaaa ctagacacaa 961 aggtacattt aaaagactta aaattttact ggatggaaca tgatggaggc tcatttttaa 1021 tgtccaaagt ggaccaagat ctcattatga ctcagaaaaa ggttgtggtt caccttgaaa 1081 ctattctagg agcctcagtt caacatgtta aactgaagaa aatgaagtac tcttttcagt 1141 tgtggatcgc aatgatgtca gcaaagggac atgatgggaa ggaacctgtg aaatttgtag 1201 atttgcttgg tgaccatggg aaacatgtca gtcctctgtg ggagttgatc aaatggtgcc 1261 tgggtctgtc agtgtacacc atcccttcca ttggactggc tttgttggaa gaaaagctca 1321 gatatagcaa tgagaaatac caaaagttta aggcagtgga agaaagcctg cgtaaagagc 1381 tggtggatat gctaggtgat gatgGTGTGT TCTTATATCC CTCACATCCC ACAGTGGCAC 1441 CTAAGCATCA TGTCCCTCTA ACACGGCCCT TCAACTTTGC TTACACAGGT GTCTTCAGTG 1501 CCCTGGGTTT GCCTGTGACC CAATGCCCAC TGGGACTGAA TGCCAAAGGA CTCCCTTTAG 1561 GCATCCAGGT TGTGGCTGGA CCCTTTAATG ATCATCTGAC CCTGGCTGTG GCCCAGTACT 1621 TGGAGAAAAC TTTTGGGGGC TGGGTCTGTC CAGGAAAGTT TTACCCAACT TTCTTGTACA 1681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1741 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1801 AGGACGAGAA CTTAAGGTCC AATAAACACG TACGCGTTAA GTCgacaatc aacctctgga 1861 ttacaaaatt tgtgaaagat t