Construct: ORF TRCN0000467438
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012850.1_s317c1
- Derived from:
- ccsbBroadEn_15590
- DNA Barcode:
- CTCGGGGCGCTAGTGCCGCCAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST8SIA1 (6489)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467438
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6489 | ST8SIA1 | ST8 alpha-N-acetyl-neuramin... | NM_003034.3 | 22.8% | 22.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 339
- ORF length:
- 249
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAGCCCCTG CGGGCGGGCC CGGCGACAAA 121 CGTCCAGAGG GGCCATGGCT GTACTGGCGT GGAAGTTCCC GCGGACCCGG CTGCCCATGG 181 GAGCCAGTGC CCTCTGTGTC GTGGTCCTCT GTTGGCTCTA CATCTTCCCC GTCTACCGGC 241 TGCCCAACGA GAAAGAGATC GTGCAGGGGG TGCTGCAACA GGGCACGGCG TGGAGGAGGA 301 ACCAGACCGC GGCCAGAGCG TTCAGCTGTG GCATTGCATG CCCAACTTTC TTGTACAAAG 361 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 421 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 481 ACGACTCGGG GCGCTAGTGC CGCCAATAAC GCGTTAAGTC gacaatcaac ctctggatta 541 caaaatttgt gaaagatt