Construct: ORF TRCN0000467441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005689.1_s317c1
- Derived from:
- ccsbBroadEn_11689
- DNA Barcode:
- ATGAAAATTGTCTACATCACGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZCCHC14 (23174)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23174 | ZCCHC14 | zinc finger CCHC-type conta... | XR_243401.3 | 2% | 1_5274del;5407_6437del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 221
- ORF end:
- 353
- ORF length:
- 132
- Sequence:
-
1 GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA 61 GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC 121 ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC 181 TAGCATCGAT TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGAGTTCCG ACACTGTCAC 241 GGCTGTGTTC CCAGCAGCGA AGGCCTCTGC GGAGCTGCCA GTCGTCTTGA ACGTGCATGG 301 GCGGCGTGTG ACATCTCCAG GGAGGCCGTC CGAAGTCGAG AATCGTCAGC TGTACCCAAC 361 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 421 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 481 ATCTTGTGGA AAGGACGAAT GAAAATTGTC TACATCACGG CCACGCGTTA AGTCgacaat 541 caacctctgg attacaaaat ttgtgaaaga tt