Construct: ORF TRCN0000467443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009229.1_s317c1
- Derived from:
- ccsbBroadEn_15868
- DNA Barcode:
- CTTTCTTCACCATTTACAATCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHDC4 (54758)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467443
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54758 | KLHDC4 | kelch domain containing 4 | NM_001184854.2 | 100% | 100% | |
| 2 | human | 54758 | KLHDC4 | kelch domain containing 4 | NM_017566.4 | 89% | 89% | 100_270del |
| 3 | human | 54758 | KLHDC4 | kelch domain containing 4 | XM_005255994.4 | 89% | 89% | 100_270del |
| 4 | human | 54758 | KLHDC4 | kelch domain containing 4 | XM_017023344.2 | 89% | 89% | 100_270del |
| 5 | human | 54758 | KLHDC4 | kelch domain containing 4 | XM_006721201.2 | 88.1% | 88% | 100_270del;507_521del |
| 6 | human | 54758 | KLHDC4 | kelch domain containing 4 | XM_006721202.2 | 88.1% | 88% | 100_270del;507_521del |
| 7 | human | 54758 | KLHDC4 | kelch domain containing 4 | XM_006721203.3 | 88.1% | 88% | 100_270del;507_521del |
| 8 | human | 54758 | KLHDC4 | kelch domain containing 4 | XM_006721204.4 | 88.1% | 88% | 100_270del;507_521del |
| 9 | human | 54758 | KLHDC4 | kelch domain containing 4 | XM_024450317.1 | 88.1% | 88% | 100_270del;507_521del |
| 10 | human | 54758 | KLHDC4 | kelch domain containing 4 | NM_001184856.2 | 83% | 83% | 100_270del;506_507ins93 |
| 11 | human | 54758 | KLHDC4 | kelch domain containing 4 | NM_001351937.2 | 73.2% | 73.2% | 0_1ins372 |
| 12 | human | 54758 | KLHDC4 | kelch domain containing 4 | NM_001351938.2 | 73.2% | 73.2% | 0_1ins372 |
| 13 | human | 54758 | KLHDC4 | kelch domain containing 4 | XM_024450318.1 | 73.2% | 73.2% | 0_1ins372 |
| 14 | human | 54758 | KLHDC4 | kelch domain containing 4 | NR_147833.2 | 69% | (many diffs) | |
| 15 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751928.2 | 68.5% | (many diffs) | |
| 16 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_933342.3 | 68.2% | (many diffs) | |
| 17 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751944.2 | 66.4% | (many diffs) | |
| 18 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751939.1 | 65.1% | 1_101del;199_290del;1583_2133del | |
| 19 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751937.1 | 64.3% | 1_42del;142_317del;1608_2158del | |
| 20 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751932.2 | 64.1% | (many diffs) | |
| 21 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751938.1 | 63.5% | 1_98del;194_341del;1636_2186del | |
| 22 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751929.2 | 63.3% | (many diffs) | |
| 23 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751927.2 | 62.7% | 1_101del;201_371del;1662_2212del | |
| 24 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751941.1 | 62.6% | 1_74del;172_373del;1666_2216del | |
| 25 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751926.2 | 62.4% | (many diffs) | |
| 26 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_429719.3 | 62.3% | (many diffs) | |
| 27 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751942.1 | 61.4% | (many diffs) | |
| 28 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751933.2 | 60.8% | (many diffs) | |
| 29 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751936.1 | 60.7% | 1_67del;167_447del;1738_2288del | |
| 30 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_002957825.1 | 59.3% | (many diffs) | |
| 31 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751930.2 | 58.5% | (many diffs) | |
| 32 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751940.1 | 58.4% | 1_71del;169_535del;1828_2378del | |
| 33 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751935.1 | 56.5% | 1_71del;171_616del;1907_2457del | |
| 34 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_933341.3 | 54% | (many diffs) | |
| 35 | human | 54758 | KLHDC4 | kelch domain containing 4 | XR_001751943.1 | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1455
- ORF length:
- 1389
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg caagaagggc aagaaggaga agaagggccg cggcgcggag aagacggccg 121 ccaagatgga gaagaaggtg tctaagcgct cgcggaagga ggagactttt ttgtataacg 181 agctctatgt ctacaatacc agaaaggaca cctggaccaa agttgacatc cccagtccac 241 ctccgaggcg ctgtgctcac caggcggtgg tagtgcctca aggtggcgga cagctgtggg 301 tctttggagg ggagtttgcc tctcccaacg gagagcagtt ctaccactac aaggatctct 361 gggtcctgca tttggccacc aagacctggg aacaagtcaa atcaacaggc ggtccttcgg 421 gtcggagtgg acatcggatg gtggcctgga agagacaatt gatcctgttt ggtggcttcc 481 atgaaagtac acgggattac atctactaca acgacgtgta tgcctttaat ctggacacct 541 tcacatggag caagctgtcc ccgtcaggga cggggcccac acccagatca ggctgccaga 601 tgtccgtcac tccccagggc ggcatcgtcg tctatggggg ctactcgaaa cagagagtta 661 agaaagacgt ggacaagggc acacggcact cagacatgtt cctgctgaag ccagaggacg 721 gaagagaaga caagtgggtt tggactcgga tgaacccttc gggggtcaag cccaccccac 781 ggtctggctt ttccgtggcc atggccccga atcaccagac actgttcttc gggggtgtct 841 gtgacgagga agaggaggag agcctgtcgg gcgagttctt caacgatctg tacttctacg 901 acgccaccag gaaccgttgg tttgagggac agctgaaggg acccaagtct gaaaagaaga 961 aacgcaggcg gGGCAGAAAA GAGGAGCCCG AAGGTGGTAG CAGGCCGGCG TGTGGGGGAG 1021 CTGGCACCCA GGGGCCTGTG CAGCTGGTCA AGGAGGTGGT GGCCGAGGAT GGCACCGTGG 1081 TCACCATTAA GCAGGTGCTC ACCGCGCCAG GCTCGGCGGG GCAGCCCCGG TCTGAGGACG 1141 AAGACAGCCT TGAGGAGGCC GGCAGCCCCG CACCTGGGCC GTGTCCACGC TCCAACGCCA 1201 TGCTGGCTGT GAAGCATGGG GTGCTCTACG TCTATGGGGG CATGTTTGAG GCCGGCGACC 1261 GCCAGGTCAC CCTCAGCGAC CTGCACTGCC TGGACCTGCA CAGGATGGAG GCGTGGAAGG 1321 CCTTGGTGGA GATGGACCCA GAAACTCAGG AGTGGCTGGA GGAGACGGAC TCGGAAGAGG 1381 ACAGTGAGGA GGTTGAGGGC GCCGAGGGTG GGGTCGACGA CGAAGACAGC GGAGAGGAGA 1441 GCGGTGCGGA GGACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1501 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGCCCGTAA CTTGAAAGTA 1561 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTTTCTTCAC CATTTACAAT 1621 CGGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt