Construct: ORF TRCN0000467467
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003854.1_s317c1
- Derived from:
- ccsbBroadEn_02979
- DNA Barcode:
- ATACCATCTTCGGATTCATGTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF11 (26994)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467467
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26994 | RNF11 | ring finger protein 11 | NM_014372.5 | 100% | 100% | |
| 2 | mouse | 29864 | Rnf11 | ring finger protein 11 | NM_013876.3 | 96.9% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaactgcctc aaaTCCCCCA CCTCGGATGA CATCTCCCTG CTTCACGAGT 121 CTCAGTCCGA CCGGGCTAGC TTTGGCGAGG GGACGGAGCC GGATCAGGAG CCGCCGCCGC 181 CATATCAGGA ACAAGTTCCA GTTCCAGTCT ACCACCCAAC ACCTAGCCAG ACTCGGCTAG 241 CAACTCAGCT GACTGAAGAG GAACAAATTA GGATAGCTCA AAGAATAGGT CTTATACAAC 301 ATCTGCCTAA AGGAGTTTAT GACCCTGGAA GAGATGGATC AGAAAAAAAG ATCCGGGAGT 361 GTGTGATCTG TATGATGGAC TTTGTTTATG GGGACCCAAT TCGATTTCTG CCGTGCATGC 421 ACATCTATCA CCTGGACTGT ATAGATGACT GGTTGATGAG ATCCTTCACG TGCCCCTCCT 481 GCATGGAGCC AGTTGATGCA GCACTGCTTT CATCCTATGA GACTAATTGC CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGAATACCAT CTTCGGATTC ATGTACGACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt