Construct: ORF TRCN0000467468
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009842.1_s317c1
- Derived from:
- ccsbBroadEn_14468
- DNA Barcode:
- GTCCAGGTGACCATGTATAAGGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCBLD1 (285761)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467468
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285761 | DCBLD1 | discoidin, CUB and LCCL dom... | NM_173674.2 | 99.8% | 99% | 1598T>A;1607_1608delAT |
| 2 | human | 285761 | DCBLD1 | discoidin, CUB and LCCL dom... | NM_001366459.2 | 97.3% | 87.3% | 1445_1446insGAAG;1572_1573ins39 |
| 3 | human | 285761 | DCBLD1 | discoidin, CUB and LCCL dom... | NM_001366460.2 | 92.1% | 80.8% | 1357_1358ins88;1488_1489ins39 |
| 4 | human | 285761 | DCBLD1 | discoidin, CUB and LCCL dom... | XM_011535773.2 | 90% | 89.6% | (many diffs) |
| 5 | human | 285761 | DCBLD1 | discoidin, CUB and LCCL dom... | XM_011535774.2 | 84.7% | 74.6% | 1_141del;1498_1499ins88;1629_1630ins39 |
| 6 | human | 285761 | DCBLD1 | discoidin, CUB and LCCL dom... | NM_001366458.2 | 75.1% | 74.8% | (many diffs) |
| 7 | human | 285761 | DCBLD1 | discoidin, CUB and LCCL dom... | XM_011535771.1 | 67.9% | 67.5% | (many diffs) |
| 8 | human | 285761 | DCBLD1 | discoidin, CUB and LCCL dom... | XM_017010775.2 | 67.9% | 67.5% | (many diffs) |
| 9 | human | 285761 | DCBLD1 | discoidin, CUB and LCCL dom... | NR_158983.2 | 42.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1683
- ORF length:
- 1617
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcccggcgcc cgcggcggcg gcgcactggc gcgggctgcc gggcggggcc 121 tcctggcttt gctgctcgcg gtctccgccc cgctccggct gcaggcggag gagctgggtg 181 atggctgtgg acacctagtg acttatcagg atagtggcac aatgacatct aagaattatc 241 ccgggaccta ccccaatcac actgtttgcg aaaagacaat tacagtacca aaggggaaaa 301 gactgattct gaggttggga gatttggata tcgaatccca gacctgtgct tctgactatc 361 ttctcttcac cagctcttca gatcaatatg gtccatactg tggaagtatg actgttccca 421 aagaactctt gttgaacaca agtgaagtaa ccgtccgctt tgagagtgga tcccacattt 481 ctggccgggg ttttttgctg acctatgcga gcagcgacca tccagattta ataacatgtt 541 tggaacgagc tagccattat ttgaagacag aatacagcaa attctgccca gctggttgta 601 gagacgtagc aggagacatt tctgggaata tggtagatgg atatagagat acctctttat 661 tgtgcaaagc tgccatccat gcaggaataa ttgctgatga actaggtggc cagatcagtg 721 tgcttcagcg caaagggatc agtcgatatg aagggattct ggccaatggt gttctttcga 781 gggatggttc cctgtcagac aagcgatttc tgtttacctc caatggttgc agcagatcct 841 tgagttttga acctgacggg caaatcagag cttcttcctc atggcagtcg gtcaatgaga 901 gtggagacca agttcactgg tctcctggcc aagcccgact tcaggaccaa ggcccatcat 961 gggcttcggg cgacagtagc aacaaccaca aaccacgaga gtggctggag atcgatttgg 1021 gggagaaaaa gaaaataaca ggaattagga ccacaggatc tacacagtcg aacttcaact 1081 tttatgttaa gagttttgtg atgaacttca aaaacaataa ttctaagtgg aagacctata 1141 aaggaattgt gaataatgaa gaaaaggtgt ttcagggtaa ctctaacttt cgggacccag 1201 tgcaaaacaa tttcatccct cccatcgtgg ccagatatgt gcgggttgtc ccccagacat 1261 GGCACCAGAG GATAGCCTTG AAGGTGGAGC TCATTGGTTG CCAGATTACA CAAGGTAATG 1321 ATTCATTGGT GTGGCGCAAG ACAAGTCAAA GCACCAGTGT TTCAACTAAG AAAGAAGATG 1381 AGACAATCAC AAGGCCCATC CCCTCGGAAG AAACATCCAC AGGAATAAAC ATTACAACGG 1441 TGGCTATTCC ATTGGTGCTC CTTGTTGTCC TGGTGTTTGC TGGAATGGGG ATCTTTGCAG 1501 CCTTTAGAAA GAAGAAGAAG AAAGGAAGTC CGTATGGATC AGCAGAGGCT CAGAAAACAG 1561 ACTGTTGGAA GCAGATTAAA TATCCCTTTG CCAGACATCA GTCAGCTGAG TTTACCATCA 1621 GCTATGATAA TGAGAAGGAG ATGACACAAA AGTTAGATCT CAACACAAGT GATGGCAGGT 1681 TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC 1741 GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG 1801 CTTTATATAT CTTGTGGAAA GGACGAGTCC AGGTGACCAT GTATAAGGGC ACGCGTTAAG 1861 TCgacaatca acctctggat tacaaaattt gtgaaagatt