Construct: ORF TRCN0000467468

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009842.1_s317c1
Derived from:
ccsbBroadEn_14468
DNA Barcode:
GTCCAGGTGACCATGTATAAGGGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCBLD1 (285761)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467468
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285761 DCBLD1 discoidin, CUB and LCCL dom... NM_173674.2 99.8% 99% 1598T>A;1607_1608delAT
2 human 285761 DCBLD1 discoidin, CUB and LCCL dom... NM_001366459.2 97.3% 87.3% 1445_1446insGAAG;1572_1573ins39
3 human 285761 DCBLD1 discoidin, CUB and LCCL dom... NM_001366460.2 92.1% 80.8% 1357_1358ins88;1488_1489ins39
4 human 285761 DCBLD1 discoidin, CUB and LCCL dom... XM_011535773.2 90% 89.6% (many diffs)
5 human 285761 DCBLD1 discoidin, CUB and LCCL dom... XM_011535774.2 84.7% 74.6% 1_141del;1498_1499ins88;1629_1630ins39
6 human 285761 DCBLD1 discoidin, CUB and LCCL dom... NM_001366458.2 75.1% 74.8% (many diffs)
7 human 285761 DCBLD1 discoidin, CUB and LCCL dom... XM_011535771.1 67.9% 67.5% (many diffs)
8 human 285761 DCBLD1 discoidin, CUB and LCCL dom... XM_017010775.2 67.9% 67.5% (many diffs)
9 human 285761 DCBLD1 discoidin, CUB and LCCL dom... NR_158983.2 42.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1683
ORF length:
1617
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gcccggcgcc cgcggcggcg gcgcactggc gcgggctgcc gggcggggcc
121tcctggcttt gctgctcgcg gtctccgccc cgctccggct gcaggcggag gagctgggtg
181atggctgtgg acacctagtg acttatcagg atagtggcac aatgacatct aagaattatc
241ccgggaccta ccccaatcac actgtttgcg aaaagacaat tacagtacca aaggggaaaa
301gactgattct gaggttggga gatttggata tcgaatccca gacctgtgct tctgactatc
361ttctcttcac cagctcttca gatcaatatg gtccatactg tggaagtatg actgttccca
421aagaactctt gttgaacaca agtgaagtaa ccgtccgctt tgagagtgga tcccacattt
481ctggccgggg ttttttgctg acctatgcga gcagcgacca tccagattta ataacatgtt
541tggaacgagc tagccattat ttgaagacag aatacagcaa attctgccca gctggttgta
601gagacgtagc aggagacatt tctgggaata tggtagatgg atatagagat acctctttat
661tgtgcaaagc tgccatccat gcaggaataa ttgctgatga actaggtggc cagatcagtg
721tgcttcagcg caaagggatc agtcgatatg aagggattct ggccaatggt gttctttcga
781gggatggttc cctgtcagac aagcgatttc tgtttacctc caatggttgc agcagatcct
841tgagttttga acctgacggg caaatcagag cttcttcctc atggcagtcg gtcaatgaga
901gtggagacca agttcactgg tctcctggcc aagcccgact tcaggaccaa ggcccatcat
961gggcttcggg cgacagtagc aacaaccaca aaccacgaga gtggctggag atcgatttgg
1021gggagaaaaa gaaaataaca ggaattagga ccacaggatc tacacagtcg aacttcaact
1081tttatgttaa gagttttgtg atgaacttca aaaacaataa ttctaagtgg aagacctata
1141aaggaattgt gaataatgaa gaaaaggtgt ttcagggtaa ctctaacttt cgggacccag
1201tgcaaaacaa tttcatccct cccatcgtgg ccagatatgt gcgggttgtc ccccagacat
1261GGCACCAGAG GATAGCCTTG AAGGTGGAGC TCATTGGTTG CCAGATTACA CAAGGTAATG
1321ATTCATTGGT GTGGCGCAAG ACAAGTCAAA GCACCAGTGT TTCAACTAAG AAAGAAGATG
1381AGACAATCAC AAGGCCCATC CCCTCGGAAG AAACATCCAC AGGAATAAAC ATTACAACGG
1441TGGCTATTCC ATTGGTGCTC CTTGTTGTCC TGGTGTTTGC TGGAATGGGG ATCTTTGCAG
1501CCTTTAGAAA GAAGAAGAAG AAAGGAAGTC CGTATGGATC AGCAGAGGCT CAGAAAACAG
1561ACTGTTGGAA GCAGATTAAA TATCCCTTTG CCAGACATCA GTCAGCTGAG TTTACCATCA
1621GCTATGATAA TGAGAAGGAG ATGACACAAA AGTTAGATCT CAACACAAGT GATGGCAGGT
1681TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC
1741GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG
1801CTTTATATAT CTTGTGGAAA GGACGAGTCC AGGTGACCAT GTATAAGGGC ACGCGTTAAG
1861TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)