Construct: ORF TRCN0000467478
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006208.1_s317c1
- Derived from:
- ccsbBroadEn_01690
- DNA Barcode:
- ACTCGGTACCATACATTACAAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNNC1 (7134)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467478
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7134 | TNNC1 | troponin C1, slow skeletal ... | NM_003280.3 | 100% | 100% | |
| 2 | mouse | 21924 | Tnnc1 | troponin C, cardiac/slow sk... | NM_009393.2 | 91% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 549
- ORF length:
- 483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgacatctac aaggctgcgg tagagcagct gacagaagag cagaaaaatg 121 agttcaaggc agccTTCGAC ATCTTCGTGC TGGGCGCTGA GGATGGCTGC ATCAGCACCA 181 AGGAGCTGGG CAAGGTGATG AGGATGCTGG GCCAGAACCC CACCCCTGAG GAGCTGCAGG 241 AGATGATCGA TGAGGTGGAC GAGGACGGCA GCGGCACGGT GGACTTTGAT GAGTTCCTGG 301 TCATGATGGT TCGGTGCATG AAGGACGACA GCAAAGGGAA ATCTGAGGAG GAGCTGTCTG 361 ACCTCTTCCG CATGTTTGAC AAAAATGCTG ATGGCTACAT CGACCTGGAT GAGCTGAAGA 421 TAATGCTGCA GGCTACAGGC GAGACCATCA CGGAGGACGA CATCGAGGAG CTCATGAAGG 481 ACGGAGACAA GAACAACGAC GGCCGCATCG ACTATGATGA GTTCCTGGAG TTCATGAAGG 541 GTGTGGAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 601 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 661 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACTCGG TACCATACAT TACAAACGAC 721 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt