Construct: ORF TRCN0000467479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013236.1_s317c1
- Derived from:
- ccsbBroadEn_09835
- DNA Barcode:
- GCAATCGAACTCTACATGATCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHRSX (207063)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467479
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 207063 | DHRSX | dehydrogenase/reductase X-l... | NM_145177.3 | 99.7% | 99.3% | 739G>C;875A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1056
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gccattgtct gcggcgcggg cggccctgcg ggtctacgcg gtaggcgccg 121 cggtgatcct ggcgcagctg ctgcggcgct gccgcggggg cttcctggag ccagttttcc 181 ccccacgacc tgaccgtgtc gctatagtga cgggagggac agatggcatt ggctattcta 241 cagcgaagca tctggcgaga cttggcatgc atgttatcat agctggaaat aatgacagca 301 aagccaaaca agttgtaagc aaaataaaag aagaaacctt gaacgacaaa gtggaatttt 361 tatactgtga cttggcttcc atgacttcca tccggcagtt tgtgcagaag ttcaagatga 421 agaagattcc tctccatgtc ctgatcaaca atgctggggt gatgatggtc cctcagagga 481 aaaccagaga tggattcgaa gaacatttcg gcctgaacta cctagggcac ttcctgctga 541 ccaaccttct cttggatacg ctgaaagagt ctgggtcccc tggccacagt gcgagggtgg 601 tcaccgtctc cTCTGCCACC CATTACGTCG CTGAGCTGAA CATGGATGAC CTTCAGAGCA 661 GTGCCTGCTA CTCACCCCAC GCAGCCTACG CCCAGAGCAA GCTGGCCCTT GTCCTGTTCA 721 CCTACCACCT CCAGCGGCTG CTGGCGGCTG AGGGAAGCCA CGTGACCGCC AACGTGGTGG 781 ACCCCGGGGT GGTCAACACG GACCTCTACA AGCACGTGTT CTGGGCCACC CGTCTGGCGA 841 AGAAGCTTCT CGGCTGGTTG CTTTTCAAGA CCCCCGATGA AGGAGCGTGG ACTTCCATCT 901 ACGCAGCAGT CACCCCAGAG CTGGAAGGAG TTGGTGGCCG TTACCTATAC AACGAGAAAG 961 AGACCAAGTC CCTCCACGTC ACCTACAACC AGAAACTGCA GCAGCAGCTG TGGTCTAAGA 1021 GTTGTGAGAT GACTGGGGTC CTTGATGTGA CCCTGTGCCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 AGCAATCGAA CTCTACATGA TCCCAACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt