Construct: ORF TRCN0000467488
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005616.1_s317c1
- Derived from:
- ccsbBroadEn_08935
- DNA Barcode:
- AATCTCTAGGTCTCCAGACCACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CERS4 (79603)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467488
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79603 | CERS4 | ceramide synthase 4 | NM_024552.3 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 2 | human | 79603 | CERS4 | ceramide synthase 4 | XM_011528290.2 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 3 | human | 79603 | CERS4 | ceramide synthase 4 | XM_011528291.2 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 4 | human | 79603 | CERS4 | ceramide synthase 4 | XM_011528292.2 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 5 | human | 79603 | CERS4 | ceramide synthase 4 | XM_011528293.2 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 6 | human | 79603 | CERS4 | ceramide synthase 4 | XM_011528294.2 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 7 | human | 79603 | CERS4 | ceramide synthase 4 | XM_011528295.2 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 8 | human | 79603 | CERS4 | ceramide synthase 4 | XM_017027303.1 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 9 | human | 79603 | CERS4 | ceramide synthase 4 | XM_017027304.1 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 10 | human | 79603 | CERS4 | ceramide synthase 4 | XM_017027305.1 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 11 | human | 79603 | CERS4 | ceramide synthase 4 | XM_024451719.1 | 99.7% | 99.4% | 717C>T;1058C>T;1136G>A |
| 12 | human | 79603 | CERS4 | ceramide synthase 4 | XR_001753762.1 | 67.2% | (many diffs) | |
| 13 | human | 79603 | CERS4 | ceramide synthase 4 | XR_002958360.1 | 61.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1248
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtccagtttc aacgagtggt tttggcagga caggttctgg ttaccaccca 121 atgtcacgtg gacagagcta gaagaccggg atggccgtgt ctacccccac ccccaggact 181 tgttggcagc cctgcccctg gcgctggtcc tcctggccat gcgccttgcc tttgagagat 241 tcattggcct gcccctgagc cggtggctgg gtgtgaggga tcagaccagg aggcaagtga 301 agcccaacgc cacgctggag aaacacttcc tcacggaagg gcacaggccc aaggagcccc 361 agctgtctct cctggccgcc cagtgtggcc tcacgctgca gcagacccag cgatggttcc 421 ggagacgccg gaaccaggat cgaccccagc tgaccaagaa gttctgtgag gccagctgga 481 ggtttctctt ctacctgtcc tccttcgtgg gcggcctctc ggtcctgtac cacgagtcat 541 ggctgtgggc accagtaatg tgctgggaca ggtacccaaa ccagactctg aagccatccc 601 tgtactggtg gtacctcttg gagctgggtt tctacctctc actgctaatc aggctgccct 661 ttgatgtcaa gcgcaaggat ttcaaggagc aggtgataca ccacttcgtg gcggtcatcc 721 tgatgacctt ctcctacagt gccaacctgc tgcgcattgg ctctctggtg ctgctgttac 781 atgattcctc tgactacctg ctggaggcct gtaagatggt caactacatg cagtatcagc 841 aagtgtgcga cgctctcttc ctcatcttct cctttgtctt cttctacacc cgactggTCC 901 TCTTTCCCAC CCAGATCCTC TACACCACAT ACTACGAGTC CATCAGCAAC AGGGGCCCCT 961 TCTTCGGCTA CTACTTCTTC AACGGGCTTC TGATGTTGCT GCAGCTGCTG CACGTGTTCT 1021 GGTCTTGCCT CATTCTGCGC ATGCTCTATA GCTTCATGAA GAAGGGCCAG ATGGAGAAGG 1081 ACATTCGTAG TGATGTAGAA GAATCAGACT CCAGTGAGGA GGTGGCGGCG GCCCAGGAAC 1141 CTCTGCAGCT AAAGAACGGG GCAGCTGGAG GGCCCAGGCC AGCCCCCACT GATGGCCCTC 1201 AGAGCCGGGT GGCCGGGCGT CTGACCAACA GGCACACAAC AGCCACATAC CCAACTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGAAATCTCT AGGTCTCCAG ACCACCAACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt