Construct: ORF TRCN0000467493
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013183.1_s317c1
- Derived from:
- ccsbBroadEn_15880
- DNA Barcode:
- CCGCAGGGTTCTCTGACCCTCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF39 (55015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467493
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55015 | PRPF39 | pre-mRNA processing factor 39 | NM_017922.4 | 80.7% | 78.3% | (many diffs) |
| 2 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516026.3 | 93.3% | 96.3% | (many diffs) |
| 3 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516027.3 | 93.3% | 96.3% | (many diffs) |
| 4 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516028.3 | 93.3% | 96.3% | (many diffs) |
| 5 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_017315120.1 | 93.3% | 96.3% | (many diffs) |
| 6 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516029.3 | 77.6% | 77.1% | (many diffs) |
| 7 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | NM_177806.3 | 74.5% | 75.5% | (many diffs) |
| 8 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516031.2 | 38.5% | 36.1% | (many diffs) |
| 9 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XR_001780462.1 | 35.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1713
- ORF length:
- 1644
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagggactg ctgcgctttg aagatcaaga ctctgcacgt ggggatcaga 121 acattgccat gttctatcca acctccaccc aaatggttta tcggcggggg cttcaggcaa 181 tacctcttag tgttgacctt tggatacatt atataaactt cttaaaagaa acattggacc 241 ctggtgatcc tgagacaaac aatacaataa gaggaacttt tgagcatgct gttctagctg 301 caggaacaga tttccgttct gacagactgt gggaaatgta tataaactgg gaaaatgagc 361 agggaaacct gagagaagtt acagctatat atgatcgtat tcttggtatt ccaacacagc 421 tgtatagtca tcattttcag agatttaaag aacatgtaca gaataatttg cctagagatc 481 ttttaactgg tgaacagttt attcagttgc gaagggaatt agcttctgta aatggtcata 541 gtggtgatga tggtcctcct ggtgatgatc taccatcggg aattgaagac ataaccgatc 601 ctgcaaagct aattacagaa atagaaaaca tgagacatag aatcattgag attcatcaag 661 aaatgtttaa ttataatgag catgaagtta gtaaaaggtg gacatttgaa gaaggtatta 721 aaagacctta ctttcatgtg aaacctttgg aaaaggcaca actaaaaaac tggaaagaat 781 acttagaatt tgaaattgaa aatgggactc atgaacgagt tgtggttctc tttgaaagat 841 gtgtcatatc atgtgccctc tatgaggagt tttggattaa gtatgccaag tacatggaaa 901 accatagcat tgaaggagtg aggcatgtct tcagcagagc ttgtactata catctcccaa 961 agaaacccat ggtgcatatg ctttgggcag cttttgagga acagcagggt aatattaatg 1021 aagccaggaa tatcttgaaa acatttgaag aatgtgttct aggattggca atggttcgtt 1081 tacgaagagt aagtttagaa cgacggcatg gaaatctgga agaagctgaa catttgcttc 1141 aggatgccat taagaatgcc aaatcaaata atgaatcttc attttatgct gtcaaactag 1201 cccggcatct tttcaaaata cagaaaaacc ttccaaaatc aagaaaggtg cttttggaag 1261 caatcgaaag agacaaagag aacacaaagt tatacctcaa tttacttgaa atggaatata 1321 gtggtgaccT CAAACAAAAT GAAGAAAATA TCCTAAATTG TTTTGACAAA GCTGTACATG 1381 GTTCATTACC TATTAAAATG AGAATTACAT TTTCTCAGAG AAAAGTGGAA TTTCTTGAAG 1441 ATTTTGGTTC CGATGTTAAT AAGCTTCTGA ATGCTTATGA TGAACATCAA ACACTCCTGA 1501 AAGAACAGGA TTCTTTAAAA AGGAAAGCAG AAAATGGATC AGAAGAACCA GAGGAAAAGA 1561 AAGCACATAC AGAAGATACA ACTTCATCAT CTACACAGAT GATTGATGGT GATTTACAGG 1621 CAAACCAAGC TGTATATAAT TATAGTGCGT GGTATCAATA CAATTATCAG AATCCTTGGA 1681 ATTATGGACA ATATTATCCT CCCCCTCCAA CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1741 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1801 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1861 GCAGGGTTCT CTGACCCTCG GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1921 ttgtgaaaga tt