Construct: ORF TRCN0000467507
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014269.1_s317c1
- Derived from:
- ccsbBroadEn_05681
- DNA Barcode:
- AGGGTCTATCAGCTTTCCTGCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CT45A3 (441519)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467507
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441519 | CT45A3 | cancer/testis antigen famil... | NM_001017435.2 | 100% | 100% | |
| 2 | human | 441519 | CT45A3 | cancer/testis antigen famil... | NM_001370148.2 | 100% | 100% | |
| 3 | human | 441519 | CT45A3 | cancer/testis antigen famil... | NM_001370149.1 | 100% | 100% | |
| 4 | human | 441521 | CT45A5 | cancer/testis antigen famil... | NM_001007551.4 | 99.8% | 99.4% | 247G>A |
| 5 | human | 541466 | CT45A1 | cancer/testis antigen famil... | NM_001017417.2 | 99.8% | 99.4% | 164C>A |
| 6 | human | 541465 | CT45A6 | cancer/testis antigen famil... | NM_001017438.2 | 99.8% | 99.4% | 247G>A |
| 7 | human | 441521 | CT45A5 | cancer/testis antigen famil... | NM_001172288.2 | 99.8% | 99.4% | 247G>A |
| 8 | human | 101060211 | CT45A7 | cancer/testis antigen famil... | NM_001291543.2 | 99.8% | 99.4% | 247G>A |
| 9 | human | 101060211 | CT45A7 | cancer/testis antigen famil... | XM_003960082.4 | 99.8% | 99.4% | 247G>A |
| 10 | human | 541465 | CT45A6 | cancer/testis antigen famil... | XM_005262425.4 | 99.8% | 99.4% | 247G>A |
| 11 | human | 541466 | CT45A1 | cancer/testis antigen famil... | XM_005278141.4 | 99.8% | 99.4% | 164C>A |
| 12 | human | 101060211 | CT45A7 | cancer/testis antigen famil... | XM_011531229.3 | 99.8% | 99.4% | 247G>A |
| 13 | human | 441521 | CT45A5 | cancer/testis antigen famil... | XM_011531341.3 | 99.8% | 99.4% | 247G>A |
| 14 | human | 541465 | CT45A6 | cancer/testis antigen famil... | XM_011531351.2 | 99.8% | 99.4% | 247G>A |
| 15 | human | 541466 | CT45A1 | cancer/testis antigen famil... | XM_011531352.1 | 99.8% | 99.4% | 164C>A |
| 16 | human | 102723737 | CT45A8 | cancer/testis antigen famil... | NM_001291535.1 | 99.2% | 98.4% | (many diffs) |
| 17 | human | 102723680 | CT45A9 | cancer/testis antigen famil... | NM_001291540.2 | 99.2% | 98.4% | (many diffs) |
| 18 | human | 102723680 | CT45A9 | cancer/testis antigen famil... | NM_001321271.1 | 99.2% | 98.4% | (many diffs) |
| 19 | human | 728911 | CT45A2 | cancer/testis antigen famil... | NM_152582.7 | 99.2% | 98.4% | (many diffs) |
| 20 | human | 728911 | CT45A2 | cancer/testis antigen famil... | XM_006724783.2 | 99.2% | 98.4% | (many diffs) |
| 21 | human | 102723680 | CT45A9 | cancer/testis antigen famil... | XM_006724796.2 | 99.2% | 98.4% | (many diffs) |
| 22 | human | 102723737 | CT45A8 | cancer/testis antigen famil... | XM_006724799.2 | 99.2% | 98.4% | (many diffs) |
| 23 | human | 102723737 | CT45A8 | cancer/testis antigen famil... | XM_011531241.2 | 99.2% | 98.4% | (many diffs) |
| 24 | human | 728911 | CT45A2 | cancer/testis antigen famil... | XM_011531389.2 | 99.2% | 98.4% | (many diffs) |
| 25 | human | 102723631 | CT45A10 | cancer/testis antigen famil... | NM_001291527.1 | 97.8% | 94.1% | (many diffs) |
| 26 | human | 102723631 | CT45A10 | cancer/testis antigen famil... | NM_001291528.1 | 97.8% | 94.1% | (many diffs) |
| 27 | human | 102723631 | CT45A10 | cancer/testis antigen famil... | NM_001291529.1 | 97.8% | 94.1% | (many diffs) |
| 28 | human | 102723631 | CT45A10 | cancer/testis antigen famil... | NM_001291530.1 | 97.8% | 94.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 633
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cgataaaaca gagaaggtgg ctgtagatcc tgaaactgtg tttaaacgtc 121 ccagggaatg tgacagtcct tcgtatcaga aaaggcagag gatggccctg ttggcaagga 181 aacaaggagc aggagacagc cttattgcag gcTCTGCCAT GTCCAAAGAA AAGAAGCTTA 241 TGACAGGACA TGCTATTCCA CCCAGCCAAT TGGATTCTCA GATTGATGAC TTCACTGGTT 301 TCAGCAAAGA TAGGATGATG CAGAAACCTG GTAGCAATGC ACCTGTGGGA GGAAACGTTA 361 CCAGCAGTTT CTCTGGAGAT GACCTAGAAT GCAGAGAAAC AGCCTCCTCT CCCAAAAGCC 421 AACGAGAAAT TAATGCTGAT ATAAAACGTA AATTAGTGAA GGAACTCCGA TGCGTTGGAC 481 AAAAATATGA AAAAATCTTC GAAATGCTTG AAGGAGTGCA AGGACCTACT GCAGTCAGGA 541 AGCGATTTTT TGAATCCATC ATCAAGGAAG CAGCAAGATG TATGAGACGA GACTTTGTTA 601 AGCACCTTAA GAAGAAACTG AAACGTATGA TTTGCCCAAC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 781 GGTCTATCAG CTTTCCTGCA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt