Construct: ORF TRCN0000467541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015592.1_s317c1
- Derived from:
- ccsbBroadEn_14669
- DNA Barcode:
- CTACTCATTGAAATTAGCGAACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXA3 (3200)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3200 | HOXA3 | homeobox A3 | NM_030661.4 | 100% | 100% | |
| 2 | human | 3200 | HOXA3 | homeobox A3 | NM_153631.2 | 100% | 100% | |
| 3 | human | 3200 | HOXA3 | homeobox A3 | XM_005249730.2 | 100% | 100% | |
| 4 | human | 3200 | HOXA3 | homeobox A3 | XM_005249731.2 | 100% | 100% | |
| 5 | human | 3200 | HOXA3 | homeobox A3 | XM_005249732.4 | 100% | 100% | |
| 6 | human | 3200 | HOXA3 | homeobox A3 | XM_006715715.2 | 100% | 100% | |
| 7 | human | 3200 | HOXA3 | homeobox A3 | XM_011515343.3 | 100% | 100% | |
| 8 | mouse | 15400 | Hoxa3 | homeobox A3 | NM_010452.3 | 88.4% | 93.4% | (many diffs) |
| 9 | mouse | 15400 | Hoxa3 | homeobox A3 | XM_011241225.2 | 88.4% | 93.4% | (many diffs) |
| 10 | mouse | 15400 | Hoxa3 | homeobox A3 | XM_017321402.1 | 88.4% | 93.4% | (many diffs) |
| 11 | mouse | 15400 | Hoxa3 | homeobox A3 | XM_017321403.1 | 88.4% | 93.4% | (many diffs) |
| 12 | mouse | 15400 | Hoxa3 | homeobox A3 | XM_017321404.1 | 88.4% | 93.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1395
- ORF length:
- 1329
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca aaaagcgacc tactacgaca gctcggcgat ctacggtggc tacccctacc 121 aggcagccaa cgggttcgct tataatgcca atcagcagcc gtacccggcg tccgccgctt 181 tgggcgccga cggcgagtac caccgacccg cctgctccct ccagtctccc tccagcgccg 241 ggggccaccc caaggcacac gaactgagtg aggcgtgcct gcgcaccctg agcgccccac 301 ctagccagcc tccaagcctg ggagagccgc ccctgcaccc gccgccgccc caggccgcgc 361 cccctgcccc acagccgcct cagcccgcac ctcagccccc tgcacctacc cctgccgcgc 421 ccccgcctcc ctcttctgcc tcccctcctc agaatgccag caacaaccct acccctgcca 481 acgcggccaa gagccccctg ctcaactcac ccacagtggc caaacaaatc ttcccctgga 541 tgaaagagtc tcgacaaaac acaaagcaga aaaccagcag ctccagctca ggcgaaagct 601 gcgctggcga caagagcccg ccggggcagg cttcgtccaa gcgcgcgcgc acggcctaca 661 cgagcgcgca gctggtggag ctggagaaag agttccactt caaccgctac ctgtgccggc 721 cgcgccgggt ggagatggcc aatctgctga acctcactga gcgccagatc aagatctggt 781 tccagaatcg ccgcatgaag tacaaaaagg atcagaaggg caagggcatg ctaacgtcat 841 cggggggcca gtctccaagt cgcagccccg tgccccccgg agccggtggc tatctgaact 901 ctatgcattc gctggtcaac agcgtcccgt atgagcccca gtcgcccccg cccttctcca 961 agccccccca gggtaccTAC GGGCTGCCCC CCGCCTCCTA CCCTGCGTCC CTGCCCAGCT 1021 GCGCACCCCC GCCACCCCCA CAGAAGCGCT ACACGGCGGC AGGGGCGGGC GCAGGGGGCA 1081 CCCCCGACTA TGACCCGCAC GCTCATGGCC TGCAGGGCAA CGGCAGCTAT GGGACCCCAC 1141 ACATACAGGG AAGCCCCGTC TTCGTGGGGG GCAGCTATGT GGAGCCCATG AGCAACTCCG 1201 GGCCAGCCCT CTTTGGTCTA ACTCACCTCC CCCACGCTGC CTCGGGCGCC ATGGACTATG 1261 GGGGTGCCGG GCCGCTGGGC AGCGGCCACC ACCACGGGCC GGGGCCTGGG GAGCCGCACC 1321 CCACCTACAC GGACCTTACC GGCCACCATC CTTCTCAGGG AAGAATTCAG GAAGCACCCA 1381 AGCTCACCCA CCTGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1441 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1501 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTACTCATTG AAATTAGCGA 1561 ACCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt