Construct: ORF TRCN0000467555
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001457.1_s317c1
- Derived from:
- ccsbBroadEn_05315
- DNA Barcode:
- GGCCCTGGCTTGCGGGGCCACTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFSD8 (256471)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467555
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256471 | MFSD8 | major facilitator superfami... | NM_001371596.1 | 100% | 100% | |
| 2 | human | 256471 | MFSD8 | major facilitator superfami... | NM_152778.3 | 100% | 100% | |
| 3 | human | 256471 | MFSD8 | major facilitator superfami... | NM_001371592.1 | 99.6% | 99.4% | 554_559delGTAGGT |
| 4 | human | 256471 | MFSD8 | major facilitator superfami... | NM_001371591.1 | 99.4% | 99.4% | 1348_1356delCAGGTAAGC |
| 5 | human | 256471 | MFSD8 | major facilitator superfami... | NM_001371593.1 | 92.6% | 92.6% | 436_437ins114 |
| 6 | human | 256471 | MFSD8 | major facilitator superfami... | NM_001371590.1 | 91.3% | 91.3% | 0_1ins135 |
| 7 | human | 256471 | MFSD8 | major facilitator superfami... | NM_001371594.1 | 90.5% | 90.5% | 551_552ins147 |
| 8 | human | 256471 | MFSD8 | major facilitator superfami... | NM_001363520.2 | 87% | 87% | 551_552ins201 |
| 9 | human | 256471 | MFSD8 | major facilitator superfami... | XM_024453982.1 | 83.9% | 83.9% | 0_1ins135;301_302ins114 |
| 10 | human | 256471 | MFSD8 | major facilitator superfami... | NM_001371595.1 | 81.8% | 81.8% | 0_1ins135;416_417ins147 |
| 11 | human | 256471 | MFSD8 | major facilitator superfami... | NM_001363521.2 | 79.7% | 79.7% | 437_438ins315 |
| 12 | human | 256471 | MFSD8 | major facilitator superfami... | XM_024453983.1 | 71% | 71% | 0_1ins135;302_303ins315 |
| 13 | human | 256471 | MFSD8 | major facilitator superfami... | XR_938717.2 | 70.3% | 1_77del;1425_1830del;1917_1918ins120 | |
| 14 | human | 256471 | MFSD8 | major facilitator superfami... | XR_001741199.1 | 67% | (many diffs) | |
| 15 | human | 256471 | MFSD8 | major facilitator superfami... | XM_005262898.3 | 64.5% | 64.2% | (many diffs) |
| 16 | human | 256471 | MFSD8 | major facilitator superfami... | XM_011531832.1 | 57.1% | 56.9% | (many diffs) |
| 17 | human | 256471 | MFSD8 | major facilitator superfami... | XR_001741198.2 | 55.6% | (many diffs) | |
| 18 | human | 256471 | MFSD8 | major facilitator superfami... | XM_017007989.1 | 51.6% | 51.3% | (many diffs) |
| 19 | human | 256471 | MFSD8 | major facilitator superfami... | XR_001741194.1 | 47.5% | 1_77del;1075_1076ins104;1528_2945del | |
| 20 | human | 256471 | MFSD8 | major facilitator superfami... | XR_001741196.1 | 40.9% | (many diffs) | |
| 21 | human | 256471 | MFSD8 | major facilitator superfami... | XR_001741197.1 | 27.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1620
- ORF length:
- 1554
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggcctgcgg aacgaaagtg aacaggagcc gctcttaggc gacacacctg 121 gaagcagaga atgggacatt ttagagactg aagagcatta taagagccga tggagatcta 181 ttaggatttt atatcttact atgtttctca gcagtgtagg gttttctgta gtgatgatgt 241 ccatatggcc atatctccaa aagattgatc cgacagctga tacaagtttt ttgggctggg 301 ttattgcttc atatagtctt ggccaaatgg tagcttcacc tatatttggt ttatggtcta 361 attatagacc aagaaaagag cctcttattg tctccatctt gatttccgtg gcagccaact 421 gcctctatgc atatctccac atcccagctt ctcataataa atactacatg ctggttgctc 481 gtggattgtt gggaattgga gcaggaaatg tagcagttgt tagatcatat actgctggtg 541 ctacttccct tcaggaaaga acaagttcca tggcaaacat aagcatgtgt caagcattag 601 gttttattct aggtccagtt tttcagactt gttttacatt ccttggagaa aaaggtgtga 661 catgggatgt gattaaactg cagataaaca tgtatacaac accagtttta cttagcgcct 721 tcctgggaat tttaaatatt attctgatcc ttgccatact aagagaacat cgtgtggatg 781 actcaggaag acagtgtaaa agtattaatt ttgaagaagc aagtacagat gaagctcagg 841 ttccccaagg aaatattgac caggttgctg ttgtggccat caatgttctg ttttttgtga 901 ctctatttat ctttgccctt tttgaaacca tcattactcc attaacaatg gatatgtatg 961 cctggactca agaacaagct gtgttatata atggcataat acttgctgct cttggggttg 1021 aagccgttgt tattttctta ggagttaagt tgctttccaa aaagattggc gagcgtgcta 1081 ttctactggg aggactcatc gttgtatggg ttggcttctt tatcttgtta ccttggggaa 1141 atcaatttcc caaaatacag tgggaagatt tgcacaataa ttcaatccct aataccacat 1201 ttggggaaat tattattggt ctttGGAAGT CTCCAATGGA AGATGACAAT GAAAGACCAA 1261 CTGGTTGCTC GATTGAACAA GCCTGGTGCC TCTACACCCC GGTGATTCAT CTGGCCCAGT 1321 TCCTTACATC AGCTGTGCTA ATAGGATTAG GCTATCCAGT CTGCAATCTT ATGTCCTATA 1381 CTCTATATTC AAAAATTCTA GGACCAAAAC CTCAGGGTGT ATACATGGGC TGGTTAACAG 1441 CATCTGGAAG TGGAGCCCGG ATTCTTGGGC CTATGTTCAT CAGCCAAGTG TATGCTCACT 1501 GGGGACCACG ATGGGCATTC AGCCTGGTGT GTGGAATAAT AGTGCTCACC ATCACCCTCC 1561 TGGGAGTGGT TTACAAAAGA CTCATTGCTC TTTCTGTAAG ATATGGGAGG ATTCAGGAAT 1621 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1681 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1741 TTTATATATC TTGTGGAAAG GACGAGGCCC TGGCTTGCGG GGCCACTGTA CGCGTTAAGT 1801 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt