Construct: ORF TRCN0000467613
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002532.1_s317c1
- Derived from:
- ccsbBroadEn_04324
- DNA Barcode:
- TTACTACGCCACGTAACAACTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HORMAD1 (84072)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467613
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84072 | HORMAD1 | HORMA domain containing 1 | NM_032132.5 | 100% | 100% | |
| 2 | human | 84072 | HORMAD1 | HORMA domain containing 1 | NM_001199829.2 | 98.2% | 98.2% | 278_279ins21 |
| 3 | human | 84072 | HORMAD1 | HORMA domain containing 1 | XM_011510054.2 | 93.6% | 93.4% | (many diffs) |
| 4 | mouse | 67981 | Hormad1 | HORMA domain containing 1 | NM_001289532.1 | 83.5% | 77% | (many diffs) |
| 5 | mouse | 67981 | Hormad1 | HORMA domain containing 1 | NM_001289534.1 | 80.1% | 73.2% | (many diffs) |
| 6 | mouse | 67981 | Hormad1 | HORMA domain containing 1 | NM_001289537.1 | 80.1% | 73.2% | (many diffs) |
| 7 | mouse | 67981 | Hormad1 | HORMA domain containing 1 | NM_026489.3 | 80.1% | 73.2% | (many diffs) |
| 8 | mouse | 67981 | Hormad1 | HORMA domain containing 1 | XM_006501954.3 | 77.4% | 71.3% | (many diffs) |
| 9 | mouse | 67981 | Hormad1 | HORMA domain containing 1 | XM_006501955.3 | 74.2% | 67.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1248
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cactgcccag ttgcagagga ctcccatgag tgcactggta tttcccaata 121 agatatcaac tgaacaccag tctttggtgt tagtgaagag gcttctagca gtttcagtat 181 cctgtatcac gtatttgagg ggaatattcc cagaatgcgc ttatggaaca agatatctag 241 atgatctttg tgtcaaaata ctgagagaag ataaaaattg cccaggatct acacagttag 301 tgaaatggat gctaggatgt tatgatgctt tacagaaaaa atatctaagg atggttgttc 361 tagctgtata cacaaaccca gaagatcctc agacaatttc agaatgttac caattcaaat 421 tcaaatacac caataatgga ccactcatgg acttcataag taaaaaccaa agcaacgaat 481 ctagcatgtt gtctactgac accaagaaag caagcattct cctcattcgc aagatttata 541 tcctaatgca aaatctgggg cctttaccta atgatgtttg tttgaccatg aaactttttt 601 actatgatga agttacaccc ccagattacc agcctcccgg ttttaaggat ggtgattgtg 661 aaggagttat atttgaaggg gaacctatgt atttaaatgt gggagaagtc tcaacacctt 721 ttcacatctt caaagtaaaa gtgaccactg agagagaacg aatggaaaat attgactcaa 781 ctatactatc accaaaacaa ataaaaacac catttcaaaa aatccTGAGG GACAAAGATG 841 TAGAAGATGA ACAGGAGCAT TATACAAGTG ATGATTTGGA CATTGAAACT AAAATGGAAG 901 AACAGGAAAA AAACCCTGCA TCTTCTGAAC TTGAAGAACC AAGTTTAGTT TGTGAGGAAG 961 ATGAAATTAT GAGGTCTAAA GAAAGTCCAG ATCTTTCTAT TTCTCATTCT CAGGTTGAGC 1021 AGTTAGTCAA TAAAACATCT GAACTTGATA TGTCTGAAAG CAAAACAAGA AGTGGAAAAG 1081 TCTTTCAGAA TAAAATGGCA AATGGAAATC AACCAGTAAA ATCTTCCAAA GAAAATCGGA 1141 AGAGAAGTCA ACATGAATCT GGGAGAATAG TCCTCCATCA CTTTGATTCT TCTAGTCAAG 1201 AGTCAGTGCC AAAAAGGAGA AAGTTTAGTG AACCAAAGGA ACATATATAC CCAACTTCCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGATTACTAC GCCACGTAAC AACTCTTACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt