Construct: ORF TRCN0000467614
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006270.1_s317c1
- Derived from:
- ccsbBroadEn_06407
- DNA Barcode:
- AGCCAACACTCATGATTCTGTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSPA1L (3305)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467614
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3305 | HSPA1L | heat shock protein family A... | NM_005527.4 | 99.7% | 99.3% | (many diffs) |
| 2 | human | 3303 | HSPA1A | heat shock protein family A... | NM_005345.6 | 80.6% | 87.5% | (many diffs) |
| 3 | human | 3304 | HSPA1B | heat shock protein family A... | NM_005346.6 | 80.5% | 87.5% | (many diffs) |
| 4 | mouse | 15482 | Hspa1l | heat shock protein 1-like | NM_013558.2 | 85.2% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1989
- ORF length:
- 1923
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tactggcaag ggaatcgcca taggcatcga cctgggcacc acctactcct 121 gtgtgggggt gttccagcac ggcaaggtgg agatcatcgc caacgaccag ggcaaccgca 181 ccacccccag ctacgtggcc ttcacagaca ccgagcggct cattggggat gcggccaaga 241 accaggtagc aatgaatccc cagaacactg tttttgatgc taaacgtctg atcggcagga 301 aatttaatga tcctgttgta caagcagata tgaaactttg gccttttcaa gtgattaatg 361 aaggaggcaa gcccaaagtc cttgtgtcct acaaagggga gaataaagct ttctaccctg 421 aggaaatctc ttcgatggta ttgactaagt tgaaggagac tgctgaggcc tttttgggcc 481 accctgtcac caatgcagtg attaccgtgc cagcctattt caatgactct caacgtcagg 541 ctactaagga tgcaggtgtg attgctggac ttaatgtgct aagaatcatc aatgagccca 601 cggctgctgc cattgcctat ggtttagata aaggaggtca aggagaacga catgtcctga 661 tttttgatct gggtggaggc acatttgatg tgtcaattct gaccatagat gatgggattt 721 ttgaggtaaa ggccactgct ggggacactc acctgggtgg ggaggacttt gacaacaggc 781 ttgtgagcca cttcgtggag gagttcaaga ggaaacacaa aaaggacatc agccagaaca 841 agcgagccgt gaggcggctg cgcaccgcct gcgagagggc caagaggacc ctgtcgtcca 901 gcacccaggc caacctagaa attgattcac tttatgaagg cattgacttc tatacatcca 961 tcaccagagc tcgatttgaa gagttgtgtg cagacctgtt taggggtacc ctggagcctg 1021 tagaaaaagc gcttcgggat gccaagatgg ataaggctaa aatccatgac attgttttag 1081 tagggggctc cacccgcatc cccaaggtgc agcggctgct tcaggactac ttcaatggac 1141 gtgatctcaa caagagcatc aaccctgatg aggccgtagc atatggggct gcggtacaag 1201 cagccatcct gatgggggac aagtctgaga aggtacagga cctgctgctg ctggacgtgg 1261 ctcccctgtc cctggggctg gagacggctg ggggcgtgat gactgccttg atgaagcgca 1321 actccaccat ccccaccaag cagacacaga ttttcaccac ctactctgac aaccaacccg 1381 gggtgctgat ccaggtgtat gagggcgaga gggccatgac aaaggacaac aacctgctgg 1441 ggcggtttga cctgactgga atccctccag cacccagggg agttcctcag atcgaggtga 1501 cgtttGACAT TGATGCCAAT GGTATTCTCA ATGTCACAGC CATGGACAAG AGCACCGGCA 1561 AGGTGAACAA GATCACCATC GCCAATGACA AGGGCCGCCT GAGCAAGGAG GAGATTGAGC 1621 GCATGGTTCT GGATGCTGAG AAATATAAAG CTGAAGATGA GGTCCAGAGG GAGAAAATTG 1681 CTGCAAAGAA TGCCTTAGAA TCCTATGCTT TTAACATGAA GAGTGTTGTG AGTGATGAAG 1741 GTTTGAAGGG CAAGATTAGT GAGTCTGATA AAAATAAAAT ATTGGATAAA TGCAACGAGC 1801 TCCTTTCGTG GCTGGAGGTC AATCAACTGG CAGAGAAAGA TGAGTTTGAT CATAAGAGAA 1861 AGGAATTGGA GCAGATGTGT AACCCTATCA TCACAAAACT CTACCAAGGA GGATGCACTG 1921 GGCCTGCCTG CGGAACAGGG TATGTGCCTG GAAGGCCTGC CACAGGCCCC ACAATTGAAG 1981 AAGTAGATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2041 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2101 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCCAA CACTCATGAT TCTGTCTTAC 2161 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt