Construct: ORF TRCN0000467619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010035.1_s317c1
- Derived from:
- ccsbBroadEn_02887
- DNA Barcode:
- TCGGATACGTCCCCTCTCCCCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLIC4 (25932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467619
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25932 | CLIC4 | chloride intracellular chan... | NM_013943.3 | 100% | 100% | |
| 2 | mouse | 29876 | Clic4 | chloride intracellular chan... | NM_013885.2 | 88.4% | 98.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gttgtcgatg ccgctgaatg ggctgaagga ggaggacaaa gagcccctca 121 tcgagctctt cgtcaaggct ggcagtgatg gtgaaagcat aggaaactgc cccttttccc 181 agaggctctt catgattctt tggctcaaag gagttgtatt tagtgtgacg actgttgacc 241 tgaaaaggaa gccagcagac ctgcagaact tggctcccgg gacccaccca ccatttataa 301 ctttcaacag tgaagtcaaa acggatgtaa ataagattga ggaatttctt gaagaagtct 361 tatgcccTCC CAAGTACTTA AAGCTTTCAC CAAAACACCC AGAATCAAAT ACTGCTGGAA 421 TGGACATCTT TGCCAAATTC TCTGCATATA TCAAGAATTC AAGGCCAGAG GCTAATGAAG 481 CACTGGAGAG GGGTCTCCTG AAAACCCTGC AGAAACTGGA TGAATATCTG AATTCTCCTC 541 TCCCTGATGA AATTGATGAA AATAGTATGG AGGACATAAA GTTTTCTACA CGTAAATTTC 601 TGGATGGCAA TGAAATGACA TTAGCTGATT GCAACCTGCT GCCCAAACTG CATATTGTCA 661 AGGTGGTGGC CAAAAAATAT CGCAACTTTG ATATTCCAAA AGAAATGACT GGCATCTGGA 721 GATACCTAAC TAATGCATAC AGTAGGGACG AGTTCACCAA TACCTGTCCC AGTGATAAGG 781 AGGTTGAAAT AGCATATAGT GATGTAGCCA AAAGACTCAC CAAGTACCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA TCGGATACGT CCCCTCTCCC CCCTACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt