Construct: ORF TRCN0000467661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013457.1_s317c1
- Derived from:
- ccsbBroadEn_07385
- DNA Barcode:
- GCTAATGGTCCTTTTATTATCGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR46 (9277)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467661
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9277 | WDR46 | WD repeat domain 46 | NM_005452.6 | 99.9% | 99.8% | 280A>G |
| 2 | human | 9277 | WDR46 | WD repeat domain 46 | NM_001164267.2 | 91% | 90.9% | 66_67ins162;118A>G |
| 3 | human | 9277 | WDR46 | WD repeat domain 46 | XR_001743743.2 | 89.9% | (many diffs) | |
| 4 | human | 9277 | WDR46 | WD repeat domain 46 | XR_002956316.1 | 82.2% | (many diffs) | |
| 5 | human | 9277 | WDR46 | WD repeat domain 46 | XM_017011484.2 | 80.8% | 75.9% | 280A>G;1734_1855del;1953_2262del |
| 6 | human | 9277 | WDR46 | WD repeat domain 46 | XM_017011485.2 | 38.1% | 34.1% | 0_1ins966;768_889del;987_1296del |
| 7 | human | 9277 | WDR46 | WD repeat domain 46 | XM_017011486.2 | 35.2% | 28.5% | (many diffs) |
| 8 | human | 9277 | WDR46 | WD repeat domain 46 | XM_017011487.2 | 35.2% | 28.5% | (many diffs) |
| 9 | mouse | 57315 | Wdr46 | WD repeat domain 46 | NM_020603.2 | 81.6% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1896
- ORF length:
- 1830
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacagccccc aagccgggca aggatgtccc gcccaagaaa gacaaacttc 121 agaccaagag aaagaaaccg cggcgatact gggaggaaga gaccgttccg accacagccg 181 gagcctctcc agggcctcct cgtaacaaga agaatcggga gctccgtcct cagagaccaa 241 aaaatgctta catcttaaag aagtctcgga tctctaagaa gcctcaggtc ccgaagaaac 301 cccgagaatg gaagaacccg gagtcccagc gcggcttgtc cggggcccaa gatccattcc 361 caggccccgc ccccgtccct gtggaagtgg tccagaagtt ctgtcgcatt gacaaatccc 421 gaaagctacc acattctaaa gccaaaactc gaagccgact tgaggtggct gaagctgagg 481 aagaggaaac aagtatcaaa gctgctcgtt ctgagctgct gcttgctgaa gaacctgggt 541 ttctggaagg ggaggatggg gaagacacag caaagatatg ccaggctgac attgtggagg 601 ctgtggacat tgcaagtgca gccaagcact ttgacttgaa tctgcggcag tttggaccct 661 acagactaaa ctactctcga actggaagac acctggcttt tggagggcgc cgaggtcatg 721 tggctgccct tgattgggta acaaagaagc ttatgtgcga gatcaacgtc atggaggcgg 781 tgcgggacat ccggtttctc cattctgagg cactgcttgc tgttgctcag aaccgctggc 841 tccacatcta tgacaatcag ggcattgagc tccactgtat ccgccgctgt gaccgagtaa 901 cacggcttga gttcctgccc ttccacttcc tcctggctac agcttcagaa acagggtttc 961 taacctacct ggatgtgtca gtggggaaga ttgtggcagc tctgaatgct cgagctgggc 1021 ggctcgatgt tatgagtcag aacccttaca atgccgtcat ccatctcgga cacagcaatg 1081 gtactgtgtc tttatggagt ccagctatga aggagccact ggcaaagatt ctctgtcatc 1141 gtggtggggt ccgggctgtg gcagtagatt ctacaggcac gtacatggcc acctctggcc 1201 tagaccacca gctgaagatc tttgacttgc gagggacgta ccagcctctg agcactcgga 1261 ccctgcccca tggagcaggg cacctggcct tctcccagag gggactgctg gtggcgggaa 1321 tgggtgacgt tgtcaacatc tgggcagggc agggcaaggc cagcccaccc tcccttgaac 1381 agccctacct cacccaccgg ctctcaggcc ctgtgcatgg ccttcagttc tgcccctttg 1441 aagatgtgct gggggtgggg cacactgggg gcatcaccag catgctggtc cctggggccg 1501 gtgagcccaa cttcgatggc ctggagagta atccatacag aagccggaag cagcgccagg 1561 agtgggaggt gaaggcccTG CTAGAGAAGG TACCTGCAGA GCTTATTTGT CTGGACCCAC 1621 GAGCCCTGGC CGAGGTGGAT GTCATCTCCC TGGAGCAGGG AAAGAAGGAG CAGATAGAGA 1681 GGCTGGGCTA TGACCCGCAG GCTAAGGCTC CCTTCCAGCC AAAGCCAAAG CAGAAGGGCC 1741 GCAGCTCCAC GGCAAGCCTG GTGAAGAGGA AGAGGAAGGT CATGGATGAG GAACACAGGG 1801 ACAAGGTCCG GCAGAGCCTT CAGCAGCAGC ATCATAAGGA GGCGAAGGCC AAGCCCACGG 1861 GGGCCCGGCC ATCTGCCCTG GACAGATTTG TGCGCTGCCC AACTTTCTTG TACAAAGTGG 1921 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1981 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2041 AGCTAATGGT CCTTTTATTA TCGAGACGCG TTAAGTCgac aatcaacctc tggattacaa 2101 aatttgtgaa agatt