Transcript: Human XM_017011487.2

PREDICTED: Homo sapiens WD repeat domain 46 (WDR46), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
WDR46 (9277)
Length:
2023
CDS:
770..2002

Additional Resources:

NCBI RefSeq record:
XM_017011487.2
NBCI Gene record:
WDR46 (9277)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017011487.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000285411 TCGATGGCCTGGAGAGTAATC pLKO_005 1185 CDS 100% 10.800 15.120 N WDR46 n/a
2 TRCN0000136646 GAAGTTCTGTCGCATTGACAA pLKO.1 49 5UTR 100% 4.950 6.930 N WDR46 n/a
3 TRCN0000137933 GACTTGAATCTGCGGCAGTTT pLKO.1 287 5UTR 100% 4.950 6.930 N WDR46 n/a
4 TRCN0000285415 GACTTGAATCTGCGGCAGTTT pLKO_005 287 5UTR 100% 4.950 6.930 N WDR46 n/a
5 TRCN0000134673 GCAATGGTACTGTGTCTTTAT pLKO.1 751 5UTR 100% 13.200 9.240 N WDR46 n/a
6 TRCN0000248480 ACCACCAGCTGAAGATCTTTG pLKO_005 876 CDS 100% 10.800 7.560 N Wdr46 n/a
7 TRCN0000275527 ATCCCGAAAGCTACCACATTC pLKO_005 70 5UTR 100% 10.800 7.560 N WDR46 n/a
8 TRCN0000137049 CAAGATCACCTGTAGGGAAAT pLKO.1 1729 CDS 100% 10.800 7.560 N WDR46 n/a
9 TRCN0000275526 CAAGATCACCTGTAGGGAAAT pLKO_005 1729 CDS 100% 10.800 7.560 N WDR46 n/a
10 TRCN0000275528 TCATGTGGCTGCCCTTGATTG pLKO_005 370 5UTR 100% 10.800 7.560 N WDR46 n/a
11 TRCN0000175428 CCAGCTGAAGATCTTTGACTT pLKO.1 880 CDS 100% 4.950 3.465 N Wdr46 n/a
12 TRCN0000137726 GAACCCTTACAATGCCGTCAT pLKO.1 716 5UTR 100% 4.050 2.835 N WDR46 n/a
13 TRCN0000136714 CTGGAGAGTAATCCATACAGA pLKO.1 1193 CDS 100% 3.000 2.100 N WDR46 n/a
14 TRCN0000137076 CAAGTATCAAAGCTGCTCGTT pLKO.1 144 5UTR 100% 2.640 1.848 N WDR46 n/a
15 TRCN0000133989 CTTCAGAAACAGGGTTTCTAA pLKO.1 619 5UTR 100% 0.563 0.394 N WDR46 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017011487.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07385 pDONR223 100% 35.2% 28.5% None (many diffs) n/a
2 ccsbBroad304_07385 pLX_304 0% 35.2% 28.5% V5 (many diffs) n/a
3 TRCN0000467661 GCTAATGGTCCTTTTATTATCGAG pLX_317 17.7% 35.2% 28.5% V5 (many diffs) n/a
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