Construct: ORF TRCN0000467686
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001942.1_s317c1
- Derived from:
- ccsbBroadEn_05175
- DNA Barcode:
- GGTGCGCCTATGCCGCGCTGGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LDHD (197257)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467686
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 197257 | LDHD | lactate dehydrogenase D | NM_153486.3 | 100% | 100% | |
| 2 | human | 197257 | LDHD | lactate dehydrogenase D | NM_194436.2 | 95.4% | 95.4% | 628_629ins69 |
| 3 | human | 197257 | LDHD | lactate dehydrogenase D | XM_024450175.1 | 92.6% | 92.6% | 1244_1363del |
| 4 | human | 197257 | LDHD | lactate dehydrogenase D | XM_024450178.1 | 88.4% | 88.4% | 628_629ins69;1175_1294del |
| 5 | human | 197257 | LDHD | lactate dehydrogenase D | XM_024450177.1 | 77.2% | 77.6% | 0_1ins169;156_384del |
| 6 | human | 197257 | LDHD | lactate dehydrogenase D | XM_024450179.1 | 73.3% | 73.4% | 0_1ins169;156_384del;688_689ins69 |
| 7 | human | 197257 | LDHD | lactate dehydrogenase D | XM_024450174.1 | 72.2% | 72.3% | 0_1ins169;156_384del;1304_1423del |
| 8 | human | 197257 | LDHD | lactate dehydrogenase D | XM_024450176.1 | 68.6% | 68.4% | (many diffs) |
| 9 | mouse | 52815 | Ldhd | lactate dehydrogenase D | NM_027570.3 | 78.4% | 81.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1587
- ORF length:
- 1521
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgactgctc aggtctgcaa cctgggagct gttcccctgg aggggctact 121 gctcccagaa ggcaaaggga gagctctgca gggacttcgt agaggctctg aaggccgtgg 181 tgggcggctc ccacgtgtcc actgccgcgg tggtccgaga gcagcacggg cgcgatgagt 241 cggtgcacag gtgcgaacct cctgatgctg tggtgtggcc ccagaacgtg gagcaggtca 301 gccggctggc agccctgtgc tatcgccaag gtgtgcccat catcccattc ggcaccggca 361 ccgggcttga gggtggcgtc tgtgctgtgc agggcggcgt ctgcgttaac ctgacgcata 421 tggaccgaat cctggagctg aaccaggagg acttctctgt ggtggtggag ccaggtgtca 481 cccgcaaagc cctcaacgcc cacctgcggg acagcggcct ctggtttccc gtggacccag 541 gcgcggacgc ctctctctgt ggcatggcgg ccaccggggc gtcggggacc aacgcggtcc 601 gctacggcac catgcgggac aacgtgctca acctggaggt ggtgctgccc gacgggcggc 661 tgctgcacac ggcgggccga ggccggcatt tccgcttcgg cttctggcca gaaatccctc 721 atcacacagc ctggtactca ccttgtgtgt ccctgggacg taggaagagt gcagccggct 781 acaacctcac ggggctcttc gtgggctccg aggggacgct gggcctcatc acagccacca 841 ccctgcgcct gcaccctgcc cctgaggcca cagtggccgc cacgtgtgcg ttccccagtg 901 tccaggctgc tgtggacagc actgtacaca tcctccaggc tgcagtgccc gtagcccgca 961 ttgagttcct ggatgaagtc atgatggatg cctgcaacag gtacagcaag ctgaattgct 1021 tagtggcgcc cacactcttc ctggagttcc atggctccca gcaggcactg gaggagcagc 1081 tgcagcgcac agaggagata gtccagcaga acggagcctc tgacttctcc TGGGCCAAGG 1141 AGGCCGAGGA GCGCAGCCGG CTTTGGACAG CACGGCACAA TGCCTGGTAC GCAGCCCTGG 1201 CCACGCGGCC AGGCTGCAAG GGCTACTCCA CGGATGTGTG TGTGCCCATC TCCCGGCTGC 1261 CGGAGATCGT GGTGCAGACC AAGGAGGATC TGAATGCCTC AGGACTCACA GGAAGCATTG 1321 TCGGGCATGT GGGTGACGGC AACTTCCACT GCATCCTGCT GGTCAACCCT GATGACGCCG 1381 AGGAACTGGG CAGGGTCAAG GCTTTTGCAG AACAGCTGGG CAGGCGGGCA CTGGCTCTCC 1441 ACGGAACGTG CACGGGGGAG CATGGCATCG GAATGGGCAA GCGGCAGCTG CTGCAGGAGG 1501 AGGTGGGCGC CGTGGGCGTG GAGACCATGC GGCAGCTCAA GGCCGTGCTA GACCCCCAAG 1561 GCCTCATGAA TCCAGGCAAA GTGCTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1621 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1681 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGTGCGCC 1741 TATGCCGCGC TGGTGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1801 aagatt