Construct: ORF TRCN0000467695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016046.1_s317c1
- Derived from:
- ccsbBroadEn_03192
- DNA Barcode:
- TCGGAGTAGTGTCCACTCGACCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL4 (51073)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467695
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51073 | MRPL4 | mitochondrial ribosomal pro... | NM_015956.3 | 100% | 100% | |
| 2 | human | 51073 | MRPL4 | mitochondrial ribosomal pro... | NM_146387.1 | 100% | 100% | |
| 3 | human | 51073 | MRPL4 | mitochondrial ribosomal pro... | NM_146388.2 | 83.5% | 76.5% | (many diffs) |
| 4 | human | 51073 | MRPL4 | mitochondrial ribosomal pro... | XM_011528045.3 | 79.3% | 69% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 999
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcagttcgtc cgggccgggg cgcgggcctg gcttcggcct accggcagcc 121 agggcctgag ttccctggcg gaagaggcag cgcgtgcgac cgagaacccg gagcaggtgg 181 cgagcgaggg tctcccggag cccgtgctgc gcaaagtcga gctcccggta cccactcatc 241 gacgcccagt gcaggcctgg gtcgagtcct tgcggggctt cgagcaggag cgcgtgggcc 301 tggccgacct gcaccccgat gttttcgcca ccgcgcccag gctggacata ctgcaccagg 361 ttgctatgtg gcagaagaac ttcaagagaa ttagctatgc caagaccaag acgagagccg 421 aggtgcgggg cggtggccgg aagccttggc cgcagaaagg cactgggcgg gcccggcatg 481 gcagcatccg ctctccgctc tggcgaggag gaggtgttgc ccatggcccc cggggCCCCA 541 CAAGTTACTA CTACATGCTG CCCATGAAGG TGCGGGCGCT GGGTCTCAAA GTGGCACTGA 601 CCGTCAAGCT GGCCCAGGAC GACCTGCACA TCATGGACTC CCTAGAGCTG CCCACCGGAG 661 ACCCACAGTA CCTGACAGAG CTGGCGCACT ACCGCCGCTG GGGGGACTCC GTACTCCTCG 721 TGGACTTAAC ACACGAGGAG ATGCCACAGA GCATCGTGGA GGCCACCTCT AGGCTTAAGA 781 CCTTCAACTT GATCCCGGCT GTTGGCCTAA ATGTGCACAG CATGCTCAAG CACCAGACGC 841 TGGTCCTGAC GCTGCCCACC GTCGCCTTCC TGGAGGACAA GCTGCTCTGG CAGGACTCAC 901 GTTACAGACC CCTCTACCCC TTCAGCCTGC CCTACAGCGA CTTCCCCCGA CCCCTACCCC 961 ACGCTACCCA GGGCCCAGCG GCCACCCCGT ACCACTGTTG CCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGATCGGAG TAGTGTCCAC TCGACCTAAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt