Construct: ORF TRCN0000467696
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000141.1_s317c1
- Derived from:
- ccsbBroadEn_10395
- DNA Barcode:
- TATCCCCCCTGGAGTCGCAGTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIR4435-2HG (541471)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467696
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_024206.2 | 28.2% | (many diffs) | |
| 2 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_146460.1 | 26.7% | (many diffs) | |
| 3 | human | 541471 | MIR4435-2HG | MIR4435-2 host gene | NR_024373.2 | 25.8% | 1_325del;482_603del | |
| 4 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_024205.3 | 22.5% | (many diffs) | |
| 5 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_146461.1 | 20.9% | (many diffs) | |
| 6 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_024204.2 | 17.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 476
- ORF length:
- 156
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGACAGACAC TGAAAATCAC GACTCATCCC CCTCCAGCAC 361 CTCTACCTGT TGCCCGCCGA TCACAGCCGG AATGCAGCTG AAAGATTCCC TGGGGCCTGG 421 TTCCAACTGC CCACTGTGGA CTCTGAGGCC TCTGCATTTG CGGGTGGTCT GCCTGTGCCC 481 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 541 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 601 TATATCTTGT GGAAAGGACG ATATCCCCCC TGGAGTCGCA GTCCTACGCG TTAAGTCgac 661 aatcaacctc tggattacaa aatttgtgaa agatt