Construct: ORF TRCN0000467711
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010908.1_s317c1
- Derived from:
- ccsbBroadEn_09768
- DNA Barcode:
- CTGTCGATAAGCGCGGCATGAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF366 (167465)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467711
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 167465 | ZNF366 | zinc finger protein 366 | NM_152625.3 | 100% | 100% | |
| 2 | mouse | 238803 | Zfp366 | zinc finger protein 366 | NM_001004149.1 | 83.9% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2301
- ORF length:
- 2232
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagaaggaa atgaagatga tcaaagacga ggatgtgcat ttcgacttgg 121 ctgtgaagaa gaccccctcc tttccccact gcctgcagcc agtggcttct cggggaaagg 181 ctccccaaag acaccccttc ccggaagctc tccgagggcc attttcccag tttcggtatg 241 aacctccccc aggagaccta gatgggttcc ccggggtctt cgaaggagca gggtctagga 301 aacggaagag catgcccaca aagatgccct ataaccaccc tgcagaagaa gtcaccctcg 361 ccctccactc agaggagaac aaaaaccacg gccttcccaa cctccctttg ctgttcccgc 421 agcccccgcg ccccaagtat gactctcaga tgatcgacct gtgcaacgtg ggcttccaat 481 tctaccgcag cctggaacac tttgggggca agcccgtcaa gcaggaaccc attaagccca 541 gcgccgtgtg gccccagcca acgcccactc cattcctgcc cacgccctac ccctactacc 601 ccaaagtcca cccgggcctc atgttcccct tcttcgtgcc ctcgtcctcg cccttcccct 661 tcagccggca caccttcctg cccaagcagc ccccggaacc tctgctgccc cggaaagccg 721 agccccagga gagcgaggag accaagcaga aggtggagag ggtggacgtg aacgtgcaga 781 tcgatgacag ctactacgtg gacgtgggcg gctcgcagaa gcgctggcag tgccccacct 841 gcgagaagtc ctacacctcc aagtacaacc tggtcaccca catcctgggc cacagtggga 901 tcaagccgca cgcgtgcacg cactgcggga agctcttcaa gcagctcagc cacctgcata 961 cccacatgct gacccaccag ggcacgcggc cccacaagtg ccaggtgtgc cacaaggcct 1021 tcacccagac cagccacctg aagcgccaca tgatgcagca cagcgaggtg aagccgcaca 1081 actgccgcgt gtgcggccgc ggctttgcct accccagcga gctcaaggcc cacgaagcca 1141 agcacgccag tgggcgcgag aacatctgtg tggagtgcgg cctcgacttc cccaccttgg 1201 cccagctgaa gagacacctc accacgcacc ggggccccat ccagtacaac tgctccgagt 1261 gcgacaagac cttccagtac ccgagccagc tgcagaacca catgatgaag cacaaggaca 1321 tccggcccta catctgctca gagtgtggca tggagtttgt gcagccgcac cacctcaagc 1381 agcactccct cacccacaag ggtgtgaagg agcataagtg tgggatttgt gggcgggagt 1441 tcaccctgct ggccaacatg aagcgacacg tgctgatcca caccaacatc cgcgcctacc 1501 agtgtcacct ctgctacaag agcttcgtgc agaagcagac cctcaaggca cacatgatcg 1561 tccactctga cgtgaagcct ttcaaatgca agctttgtgg gaaggaattc aaccggatgc 1621 acaacctgat gggccacatg cacctgcact cagacagcaa acccttcaag tgcctctatt 1681 gcccaagcaa attcaccctg aaggggaacc tgacacgcca catgaaagtc aagcatggag 1741 tcatggagcg gggccttcat tcccaaggtc tgggaagggg gagaatcgcc ctggcacaga 1801 cagccggtgt cctgaggagt ctggagcagg aggagccctt tgacctctct cagaagcgcc 1861 gggccaaggt gccggtgttc cagTCAGACG GGGAGAGTGC CCAGGGCAGC CACTGCCACG 1921 AGGAGGAAGA GGAGGATAAC TGCTACGAGG TGGAGCCCTA CAGCCCTGGC CTGGCCCCCC 1981 AGAGCCAGCA GCTCTGCACA CCCGAGGATC TGTCCACCAA GTCGGAGCAC GCCCCCGAGG 2041 TGCTGGAGGA AGCCTGCAAG GAGGAGAAGG AGGATGCATC CAAGGGAGAA TGGGAGAAGA 2101 GGAGCAAGGG TGACCTTGGG GCAGAGGGCG GCCAGGAGAG AGACTGTGCC GGCAGAGATG 2161 AGTGTCTCAG TCTCAGGGCT TTTCAGAGTA CCCGGCGGGG CCCCTCTTTT TCTGATTACT 2221 TATACTTCAA GCACAGAGAT GAGAGTTTGA AAGAATTACT GGAGAGGAAA ATGGAAAAAC 2281 AAGCAGTGCT TTTAGGTATC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2341 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2401 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTGT CGATAAGCGC 2461 GGCATGAACC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt