Construct: ORF TRCN0000467721
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012115.1_s317c1
- Derived from:
- ccsbBroadEn_05660
- DNA Barcode:
- AGCGGGGGCTGCTCACCACCGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C2orf27B (408029)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467721
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29798 | C2orf27A | chromosome 2 open reading f... | NM_001354871.2 | 95.3% | 93.7% | (many diffs) |
| 2 | human | 29798 | C2orf27A | chromosome 2 open reading f... | NM_013310.5 | 95.3% | 93.7% | (many diffs) |
| 3 | human | 29798 | C2orf27A | chromosome 2 open reading f... | NM_001354872.2 | 64.5% | 64.5% | 0_1ins222 |
| 4 | human | 408029 | C2orf27B | chromosome 2 open reading f... | NR_161366.1 | 47.3% | 1_493del;1121_1325del | |
| 5 | human | 29798 | C2orf27A | chromosome 2 open reading f... | NR_149013.2 | 26.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgtcaggcct gagagtggag agcaggggcc agagacacta gctcctgctt 121 caggtgcaga aatacagcga tttccagtgc cagctgttga gccagtgcca gcaccagggg 181 cagattcccc tccagggaca gcgctggagc tagaggaagc tccagagccc tcctgccgct 241 gccctgggac tgcccaggac cagcccagtg aggagctgcc tgacttcatg gcacctcctg 301 tagagccacc ggccTCAGCC CTGGAGCTGA AAGTGTGGCT GGAGCTAGAG GTGGCAGAGA 361 GGGGTGGCCA GCACAGCTCC AGCCAGCAGC TCCCACACTG CTCCCAGTCC TGGGCACAGT 421 GGAAGCTATG GAGGCAGAGA CCAGGGTTTG CAATCTGGGC TCCTCTGCCT CACTGGAGAG 481 GGACTTCTCT CATTCAGCAG AGCAGCAGCC CTGCTGCTGA AGGGCCTGCT GCTACTGCTG 541 CTGGGGCTGT TTGCCTGCCT GCAGGAGGTG CTGGAGAGCA AGAAAAGGAG CCTGTGAGCA 601 GGGGTTCCAG CAGGTCCTCC TGCTCCCAGA GGCGACCTCC TCCTCCAGGC ATGGAGGTTT 661 GCCCTCAGCT GGGCATCTGG GCCATTTGCC CCTACCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 841 CGGGGGCTGC TCACCACCGA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt