Construct: ORF TRCN0000467725
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004885.1_s317c1
- Derived from:
- ccsbBroadEn_14078
- DNA Barcode:
- ACACATTCATGTACTTGTTACGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JADE2 (23338)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467725
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23338 | JADE2 | jade family PHD finger 2 | NM_015288.6 | 31% | 30.8% | 1_1632del;2221G>A;2369C>T |
| 2 | human | 23338 | JADE2 | jade family PHD finger 2 | NM_001289984.2 | 31% | 30.8% | 1_1635del;2224G>A;2372C>T |
| 3 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_024446007.1 | 29.6% | 29.4% | 1_1746del;2335G>A;2483C>T |
| 4 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_017009282.1 | 29.4% | 29.2% | 1_1761del;2350G>A;2498C>T |
| 5 | human | 23338 | JADE2 | jade family PHD finger 2 | NM_001308143.2 | 29.4% | 29.2% | 1_1764del;2353G>A;2501C>T |
| 6 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_005271945.2 | 29.4% | 29.2% | 1_1764del;2353G>A;2501C>T |
| 7 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_005271946.2 | 29.4% | 29.2% | 1_1764del;2353G>A;2501C>T |
| 8 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_005271948.4 | 29.4% | 29.2% | 1_1764del;2353G>A;2501C>T |
| 9 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_011543291.3 | 29.4% | 29.2% | 1_1764del;2353G>A;2501C>T |
| 10 | human | 23338 | JADE2 | jade family PHD finger 2 | NM_001289985.2 | 28.8% | 28.7% | 1_1812del;2401G>A;2549C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 804
- ORF length:
- 738
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catgagcgag tggccactga acaatgggca ccgcgaggac cctgctccag 121 ggctgctgtc agaggaactg ctgcaggacg aggagacact gctcagcttc atgcgggacc 181 cctcgctgcg acctggtgac cctgctagga aggcccgagg ccgcacccgc ctgcctgcca 241 agaagaaacc accaccacca ccaccgcagg acgggcctgg ttcacggacg actccagaca 301 aagcccccaa gaagacctgg ggccaggatg caggcagtgg caaggggggt caagggccac 361 ctaccaggaa gccaccacgt cggacatctt cTCACTTGCC GTCCAGCCCT GCAGCCGGGG 421 ACTGTCCCAT CCTAGCCACC CCTGAAAGCC CCCCGCCACT GGCCCCTGAG ACCCCGGACG 481 AGGCAGCCTC AGTAGCTGCT GACTCAGATG TCCAAGTGCC TGGCCCTGCA GCAAGCCCTA 541 AGCCTTTGGG CCGGCTCCGG CCACCCCGCG AGAGCAAGGT AACCCGGAGA TTGCCGGGTG 601 CCAGGCCTGA TGCTGGGATG GGACCACCTT CAGCTGTGGC TGAGAGGCCC AAGATCAGCC 661 TGCATTTTGA CACTGAGACT GATGGCTACT TCTCTGATGG GGAGATGAGC GACTCAGATG 721 TAGAGGCCGA GGACGGTGGG GTGCAGCGGG GTCCCCGGGA GGCAGGGGCA GAGGAGGTGG 781 TCCGCATGGG CGTACTGGCC TTCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 841 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 901 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CACATTCATG 961 TACTTGTTAC GCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1021 att