Construct: ORF TRCN0000467729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005746.1_s317c1
- Derived from:
- ccsbBroadEn_09785
- DNA Barcode:
- CAGGTACTCACGCACTCCACGGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CLYBL (171425)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 171425 | CLYBL | citrate lyase beta like | NM_206808.3 | 99.8% | 98.8% | 721A>G;1012A>T |
| 2 | human | 171425 | CLYBL | citrate lyase beta like | NR_104592.2 | 39.4% | (many diffs) | |
| 3 | mouse | 69634 | Clybl | citrate lyase beta like | NM_029556.3 | 83.9% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1077
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctacgtctg ctgcggaggg cggcgcgcgg agctgcggcg gcggcgctgc 121 tgaggctgaa agcgtctcta gcagctgata tccccagact tggatatagt tcctcatccc 181 atcacaagta catcccccgg agggcagtgc tttatgtacc tggaaatgat gaaaagaaaa 241 taaagaagat tccatccctg aatgtagatt gtgcagtgct cgactgtgag gatggagtgg 301 ctgcaaacaa aaagaatgaa gctcgactga gaattgtaaa aactcttgaa gacattgatc 361 tgggccctac tgaaaaatgt gtgagagtca actcagtttc cagtggtctg gcggaagaag 421 acctagagac ccttttgcaa tcccgggtcc ttccttccag cctgatgcta ccaaaggtgg 481 aaagtcctga agaaatccag tggtttgcag acaaattttc attccactta aaaggccgaa 541 aacttgaaca accaatgaat ttaatccctt ttgtggaaac tgcaatgggt ttgctcaatt 601 ttaaggcagt gtgtgaagaa acccTGAAGG TCGGGCCTCA AGTAGGTCTC TTTCTAGATG 661 CAGTCGTTTT TGGAGGAGAA GACTTTCGAG CCAGCATAGG TGCAACAAGT AGTAAAGAAA 721 CCCTGGATAT TCTCTACGCC CGGCAAAAGA TTGTTGTCAT AGCGAAAGCC TTTGGTCTCC 781 AAGCCGTAGA TCTGGTGTAC ATTGACTTTC GAGATGGAGC TGGGCTGCTT AGACAGTCAC 841 GAGAAGGAGC CGCCATGGGC TTCACTGGTA AGCAGGTGAT TCACCCTAAC CAAATTGCCG 901 TGGTCCAGGA GCAGTTTTCT CCTTCCCCTG AAAAAATTAA GTGGGCTGAA GAACTGATTG 961 CTGCCTTTAA AGAACATCAA CAATTAGGAA AGGGGGCCTT TACTTTCCAA GGGAGTATGA 1021 TCGACATGCC ATTACTGAAG CAGGCCCAGA ACACTGTTAC GCTTGCCACC TCCATCTAGG 1081 AAAAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAGGTACTC ACGCACTCCA CGGTAACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt