Construct: ORF TRCN0000467754
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015177.1_s317c1
- Derived from:
- ccsbBroadEn_07054
- DNA Barcode:
- CCGCCCATCGTGCAGTATACTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467754
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7036 | TFR2 | transferrin receptor 2 | NM_003227.4 | 99.9% | 99.8% | 458T>C |
| 2 | human | 7036 | TFR2 | transferrin receptor 2 | NM_001206855.2 | 78.6% | 78.6% | 0_1ins513 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2472
- ORF length:
- 2403
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcggctt tggggtctat tccagagagc gcaacaactg tccccaagat 121 cctctcagac cgtctaccag cgtgtggaag gcccccggaa agggcacctg gaggaggaag 181 aggaagacgg ggaggagggg gcggagacat tggcccactt ctgccccatg gagctgaggg 241 gccctgagcc cctgggctct agacccaggc agccaaacct cattccctgg gcggcagcag 301 gacggagggc tgccccctac ctggtcctga cggccctgct gatcttcact ggggccttcc 361 tactgggcta cgtcgccttc cgagggtcct gccaggcgtg cggagactct gtgttggtgg 421 tcagtgagga tgtcaactat gagcctgacc tggatttcca ccagggcaga ctctactgga 481 gcgacctcca ggccatgttc ctgcagttcc tgggggaggg gcgcccggag gacaccatca 541 ggcaaaccag ccttcgggaa cgggtggcag gctcggccgg gatggccgct ctgactcagg 601 acattcgcgc ggcgctctcc cgccagaagc tggaccacgt gtggaccgac acgcactacg 661 tggggctgca attcccggat ccggctcacc ccaacaccct gcactgggtc gatgaggccg 721 ggaaggtcgg agagcagctg ccgctggagg accctgacgt ctactgcccc tacagcgcca 781 tcggcaacgt cacgggagag ctggtgtacg cccactacgg gcggcccgaa gacctgcagg 841 acctgcgggc caggggcgtg gatccagtgg gccgcctgct gctggtgcgc gtgggggtga 901 tcagcttcgc ccagaaggtg accaatgctc aggacttcgg ggctcaagga gtgctcatat 961 acccagagcc agcggacttc tcccaggacc cacccaagcc aagcctgtcc agccagcagg 1021 cagtgtatgg acatgtgcac ctgggaactg gagaccccta cacacctggc ttcccttcct 1081 tcaatcaaac ccagttccct ccagttgcat catcaggcct tcccagcatc ccagcccagc 1141 ccatcagtgc agacattgcc tcccgcctgc tgaggaagct caaaggccct gtggcccccc 1201 aagaatggca ggggagcctc ctaggctccc cttatcacct gggccccggg ccacgactgc 1261 ggctagtggt caacaatcac aggacctcca cccccatcaa caacatcttc ggctgcatcg 1321 aaggccgctc agagccagat cactacgttg tcatcggggc ccagagggat gcatggggcc 1381 caggagcagc taaatccgct gtggggacgg ctatactcct ggagctggtg cggacctttt 1441 cctccatggt gagcaacggc ttccggcccc gcagaagtct cctcttcatc agctgggacg 1501 gtggtgactt tggaagcgtg ggctccacgg agtggctaga gggctacctc agcgtgctgc 1561 acctcaaagc cgtagtgtac gtgagcctgg acaacgcagt gctgggggat gacaagtttc 1621 atgccaagac cagccccctt ctgacaagtc tcattgagag tgtcctgaag caggtggatt 1681 ctcccaacca cagtgggcag actctctatg aacaggtggt gttcaccaat cccagctggg 1741 atgctgaggt gatccggccc ctacccatgg acagcagtgc ctattccttc acggcctttg 1801 tgggagtccc tgccgtcgag ttctccttta tggaggacga ccaggcctac ccattcctgc 1861 acacaaagga ggacacttat gagaacctgc ataaggtgct gcaaggccgc ctgcccgccg 1921 tggcccaggc cgtggcccag ctcgcagggc agctcctcat ccggctcagc cacgatcgcc 1981 tgctgcccct cgacttcggc cgctacgggg acgtcgtcct caggcacatc gggaacctca 2041 acgagttctc tggggaccTC AAGGCCCGCG GGCTGACCCT GCAGTGGGTG TACTCGGCGC 2101 GGGGGGACTA CATCCGGGCG GCGGAAAAGC TGCGGCAGGA GATCTACAGC TCGGAGGAGA 2161 GAGACGAGCG ACTGACACGC ATGTACAACG TGCGCATAAT GCGGGTGGAG TTCTACTTCC 2221 TTTCCCAGTA CGTGTCGCCA GCCGACTCCC CGTTCCGCCA CATCTTCATG GGCCGTGGAG 2281 ACCACACGCT GGGCGCCCTG CTGGACCACC TGCGGCTGCT GCGCTCCAAC AGCTCCGGGA 2341 CCCCCGGGGC CACCTCCTCC ACTGGCTTCC AGGAGAGCCG TTTCCGGCGT CAGCTAGCCC 2401 TGCTCACCTG GACGCTGCAA GGGGCAGCCA ATGCGCTTAG CGGGGATGTC TGGAACATTG 2461 ATAACAACTT CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2521 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2581 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCG CCCATCGTGC AGTATACTTA 2641 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t