Construct: ORF TRCN0000467763
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005141.1_s317c1
- Derived from:
- ccsbBroadEn_02273
- DNA Barcode:
- TTCATAATAGTCATATGCATTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMMECR1 (9949)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467763
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9949 | AMMECR1 | AMMECR nuclear protein 1 | NM_001025580.2 | 100% | 100% | |
| 2 | human | 9949 | AMMECR1 | AMMECR nuclear protein 1 | NM_015365.3 | 88.8% | 88.8% | 474_584del |
| 3 | human | 9949 | AMMECR1 | AMMECR nuclear protein 1 | NM_001171689.2 | 51.9% | 51.9% | 0_1ins369;105_215del |
| 4 | mouse | 56068 | Ammecr1 | Alport syndrome, mental ret... | NM_019496.3 | 78.4% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 954
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgggttgc tgcggggtga agaagcagaa actgtccagt tcgcccccct 121 ctggctcggg tggcggtggt ggcgcctcct cctcctccca ctgcagcgga gagagccagt 181 gccgagctgg ggagctggga ctaggaggcg ccggtacgcg gctcaacggg ctgggaggtc 241 taaccggagg aggtagcggc agcggctgta ccctctctcc cccccagggc tgcggcggcg 301 gcggcggggg gatcgccctg tcgccacctc cgagctgcgg agtggggacc ctactttcta 361 ccccggccgc cgccacctct tcctcaccct cctcatcgtc cgccgcctcg tcctcatcgc 421 cgggctcccg gaagatggtg gtgtcagcag agatgtgctg cttttgcttc gatgtgctct 481 actgtcacct gtatGGATAC CAGCAGCCCC GGACCCCCCG ATTCACCAAC GAGCCCTATG 541 CCCTTAAAGA TAGCCGTTTT CCCCCAATGA CAAGGGATGA GCTGCCACGG CTTTTCTGCT 601 CAGTGTCTCT GCTCACTAAC TTTGAAGATG TCTGTGATTA TTTGGACTGG GAGGTGGGTG 661 TACATGGCAT TAGAATAGAA TTCATCAATG AAAAAGGATC AAAACGCACC GCCACCTACC 721 TACCGGAGGT TGCAAAGGAG CAAGGATGGG ACCATATACA GACCATAGAC TCCTTATTGA 781 GGAAAGGAGG ATACAAAGCT CCGATTACTA ATGAATTCAG GAAAACCATA AAACTGACCA 841 GGTATCGTAG TGAAAAGATG ACCCTGAGCT ATGCTGAATA CCTTGCTCAT CGCCAGCATC 901 ATCATTTCCA AAATGGCATT GGGCATCCCC TTCCGCCATA CAACCATTAT TCCTGCCCAA 961 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1021 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1081 TATCTTGTGG AAAGGACGAT TCATAATAGT CATATGCATT CGGACGCGTT AAGTCgacaa 1141 tcaacctctg gattacaaaa tttgtgaaag att